Laundry in a washing machine as a mediator of secondary and tertiary DNA transfer
| Autor: | Lev Voskoboinik, Ayeleth Reshef, Merav Amiel, Ron Gafny, Mark Barash |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Laundry Context (language use) Polymerase Chain Reaction Clothing Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound Forensic dna 0302 clinical medicine Humans 030216 legal & forensic medicine Laundering Chemistry business.industry DNA Pulp and paper industry DNA Fingerprinting Biological materials Biotechnology 030104 developmental biology DNA profiling STR analysis Female business Microsatellite Repeats |
| Zdroj: | International Journal of Legal Medicine. 132:373-378 |
| ISSN: | 1437-1596 0937-9827 |
| Popis: | The aim of this work was to investigate the possibility of secondary and tertiary DNA transfer during laundry. The modes of transfer tested were mixed and separate laundry of worn and unworn garments in household and public washing machines. In addition, the possibility of a background DNA carry-over from a washing machine's drum was investigated. In the mixed (worn and unworn garments washed together) laundry experiment, 22% of samples from new unworn socks with no traceable DNA prior to experiment produced DNA profiles post-laundry. In the tertiary DNA transfer experiment performed in a public washing machine (unworn garments only), no detectable DNA profiles were observed. Samples collected from the internal drum of 25 washing and drying machines did not produce detectable STR profiles. The implications of these results are discussed in the context of forensic DNA casework analysis. Graphical Abstract ᅟA real-life scenario of secondary DNA transfer between worn and unworn garments during machine washing has been evaluated. Experiments demonstrated this scenario is possible (22% of samples) and may in fact result in high quality DNA profiles. On the contrary, testing washing machine's interior for deposition of biological material between separate washing cycles to serve as a mediator of tertiary DNA transfer resulted in no DNA profiles. |
| Databáze: | OpenAIRE |
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