Interferon-γ downregulates the proliferative response of hapten-specific B cells stimulated by antigen and cytokines
| Autor: | Beverley L. Pike, Lothar Vogel |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
0301 basic medicine
Cell number Immunology Cell Down-Regulation Lymphocyte Activation Interferon-gamma Mice 03 medical and health sciences 0302 clinical medicine Immune system Antigen medicine Animals Immunology and Allergy Interferon gamma Viability assay Antigens Cells Cultured B-Lymphocytes Chemistry Cell Biology Molecular biology Proliferative response 030104 developmental biology medicine.anatomical_structure Immunoglobulin M Antibody Formation Mice Inbred CBA Cytokines Haptens Hapten 030215 immunology medicine.drug |
| Zdroj: | Immunology and Cell Biology. 73:52-56 |
| ISSN: | 0818-9641 |
| Popis: | IFN-gamma plays a role in many aspects of cellular interactions, both positive and negative. Among its functions during the immune response, the antagonistic effects of IFN-gamma and IL-4 are well documented. Observations in our laboratory suggested that IFN-gamma could also interfere with the activation of single, antigen-specific B cells by antigen and other cytokines. Closer examination revealed that IFN-gamma reduced the number of proliferating cell clones in response to antigen and a variety of cytokines, alone or in combination. Cell viability remained at the initial level and the cells were still able to produce Ig, albeit to a lesser extent than in the absence of IFN-gamma. On the other hand, the frequency of IgM secreting clones was not affected, whereas the total amount of secreted IgM was lower in the presence of IFN-gamma, probably due to the reduced cell number and a decrease in Ig production. In addition, proliferation was prevented when B cells were pre-incubated with IFN-gamma and then stimulated by other cytokines. Kinetic studies revealed that INF-gamma had to be present from the onset of culture because delayed addition did not inhibit the proliferation of the B cells. After its initial action, IFN-gamma could be removed without abolishing the negative signal for proliferation. From these results it can be concluded that IFN-gamma transmits a signal that causes B cells to stop proliferating and prevents them from forming large clones. |
| Databáze: | OpenAIRE |
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