Identification of a new human coronavirus
| Autor: | Joke Spaargaren, Krzysztof Pyrc, Pauline M. E. Wertheim-van Dillen, Ben Berkhout, Katja C. Wolthers, Wilma Vermeulen-Oost, Jos Kaandorp, Lia van der Hoek, Maarten F. Jebbink, Ron J. M. Berkhout |
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| Přispěvatelé: | AII - Amsterdam institute for Infection and Immunity, Medical Microbiology and Infection Prevention |
| Rok vydání: | 2004 |
| Předmět: |
Adult
Human coronavirus NL63 Human coronavirus 229E viruses Molecular Sequence Data Population Genome Viral medicine.disease_cause Alphacoronavirus Article General Biochemistry Genetics and Molecular Biology Virus medicine Humans Human coronavirus OC43 education Aged DNA Primers Coronavirus education.field_of_study Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Infant virus diseases General Medicine Middle Aged biology.organism_classification Virology ddc: 610 Human coronavirus HKU1 |
| Zdroj: | Nature Medicine Nature medicine, 10(4), 368-373. Nature Publishing Group International Conference on SARS-one year after the (first) outbreak; 20040508-20040511; Lübeck; DOC04sars7.01 /20040526/ |
| ISSN: | 1546-170X 1078-8956 |
| Popis: | Three human coronaviruses are known to exist: human coronavirus 229E (HCoV-229E), HCoV-OC43 and severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). Here we report the identification of a fourth human coronavirus, HCoV-NL63, using a new method of virus discovery. The virus was isolated from a 7-month-old child suffering from bronchiolitis and conjunctivitis. The complete genome sequence indicates that this virus is not a recombinant, but rather a new group 1 coronavirus. The in vitro host cell range of HCoV-NL63 is notable because it replicates on tertiary monkey kidney cells and the monkey kidney LLC-MK2 cell line. The viral genome contains distinctive features, including a unique N-terminal fragment within the spike protein. Screening of clinical specimens from individuals suffering from respiratory illness identified seven additional HCoV-NL63-infected individuals, indicating that the virus was widely spread within the human population. Supplementary information The online version of this article (doi:10.1038/nm1024) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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