Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls
| Autor: | Danniele L. Vale, Rachel B. Caligiorne, Daniel Dias, Lourena E. Costa, Miguel A. Chávez-Fumagalli, Grasiele S.V. Tavares, Abel Martínez-Rodrigo, Bethina T. Steiner, Ricardo Andrez Machado-de-Ávila, Antônio Lúcio Teixeira, Danielle F. de Magalhães-Soares, Patrícia A.F. Ribeiro, Eduardo A.F. Coelho, Daniela P. Lage, Amanda S. Machado, Fernanda F. Ramos, Julia A.G. Silveira |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Antigenicity Immunogen 030106 microbiology Protozoan Proteins Antibodies Protozoan Antigens Protozoan Sensitivity and Specificity Epitope 03 medical and health sciences Dogs Immunogenicity Vaccine 0302 clinical medicine medicine Animals Humans Serologic Tests Dog Diseases 030212 general & internal medicine Leishmania infantum Cells Cultured biology business.industry Immunogenicity General Medicine biology.organism_classification medicine.disease Leishmania Recombinant Proteins Infectious Diseases Visceral leishmaniasis Immunology Leukocytes Mononuclear Cytokines Epitopes B-Lymphocyte Leishmaniasis Visceral business Immunogenicity Study |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 95:134-143 |
| ISSN: | 0732-8893 |
| Popis: | The diagnosis of visceral leishmaniasis (VL) presents problems due to the toxicity and/or high cost of drugs. In addition, no vaccine exists to protect against human disease. In this study, the antigenicity and immunogenicity of amastin protein were evaluated in L. infantum–infected dogs and humans. For the diagnosis, besides the recombinant protein, 1 linear B-cell epitope was synthetized and evaluated in serological assays. Results showed high sensitivity and specificity values to detect the disease when both antigens were employed against a canine and human serological panel. By contrast, when using rA2 and a soluble Leishmania antigenic preparation, sensitivity and specificity values proved to be lower. A preliminary immunogenicity study showed that the amastin protein induced high IFN-γ and low IL-10 production in stimulated PBMC derived from treated VL patients and healthy subjects, thus suggesting a potential use of this protein as an immunogen to protect against human disease. |
| Databáze: | OpenAIRE |
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