Small G Rac1 is involved in replication cycle of dengue serotype 2 virus in EAhy926 cells via the regulation of actin cytoskeleton
| Autor: | Jing An, Na Wu, Dongying Fan, Wenli Yan, Ziyang Sheng, Jing Zhang, Na Gao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
rac1 GTP-Binding Protein
0301 basic medicine Cytochalasin D vascular endothelial cells 030106 microbiology Dengue virus Biology Virus Replication Microfilament medicine.disease_cause Membrane Fusion General Biochemistry Genetics and Molecular Biology Cell Line Dengue fever Pathogenesis small Rho GTPase Rac1 03 medical and health sciences chemistry.chemical_compound Aedes Environmental Science(all) Depsipeptides medicine Animals Actin General Environmental Science dengue virus Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) medicine.disease Actin cytoskeleton Cell biology Actin Cytoskeleton 030104 developmental biology chemistry Viral replication General Agricultural and Biological Sciences actin Research Paper |
| Zdroj: | Science China. Life Sciences |
| ISSN: | 1869-1889 1674-7305 |
| DOI: | 10.1007/s11427-016-5042-5 |
| Popis: | Bleeding is a clinical characteristic of severe dengue and may be due to increased vascular permeability. However, the pathogenesis of severe dengue remains unclear. In this study, we showed that the Rac1-microfilament signal pathway was involved in the process of DENV serotype 2 (DENV2) infection in EAhy926 cells. DENV2 infection induced dynamic changes in actin organization, and treatment with Cytochalasin D or Jasplakinolide disrupted microfilament dynamics, reduced DENV2 entry, and inhibited DENV2 assembly and maturation. Rac1 activities decreased during the early phase and gradually increased by the late phase of infection. Expression of the dominant-negative form of Rac1 promoted DENV2 entry but inhibited viral assembly, maturation and release. Our findings demonstrated that Rac1 plays an important role in the DENV2 life cycle by regulating actin reorganization in EAhy926 cells. This finding provides further insight into the pathogenesis of severe dengue. |
| Databáze: | OpenAIRE |
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