Dependence of Arbuscular-Mycorrhizal Fungi on Their Plant Host for Palmitic Acid Synthesis
| Autor: | Martin Trépanier, Tyler J. Avis, Serge Gagné, Guillaume Bécard, C. Willemot, Peter Moutoglis, Jacques-André Rioux |
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| Rok vydání: | 2005 |
| Předmět: |
Sucrose
Hypha Palmitic Acid Mycology Acetates Biology Applied Microbiology and Biotechnology Palmitic acid chemistry.chemical_compound Gene Expression Regulation Fungal Mycorrhizae Onions Botany Carbon Radioisotopes Mycorrhiza Symbiosis Mycelium Fatty acid synthesis chemistry.chemical_classification Ecology Fatty acid metabolism Fatty Acids fungi Fungi Fatty acid Spores Fungal biology.organism_classification Culture Media Daucus carota Spore chemistry Biochemistry Fatty Acid Synthases Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 71:5341-5347 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Lipids are the major form of carbon storage in arbuscular-mycorrhizal fungi. We studied fatty acid synthesis by Glomus intraradices and Gigaspora rosea . [ 14 C]Acetate and [ 14 C]sucrose were incorporated into a synthetic culture medium to test fatty acid synthetic ability in germinating spores ( G. intraradices and G. rosea ), mycorrhized carrot roots, and extraradical fungal mycelium ( G. intraradices ). Germinating spores and extraradical hyphae could not synthesize 16-carbon fatty acids but could elongate and desaturate fatty acids already present. The growth stimulation of germinating spores by root exudates did not stimulate fatty acid synthesis. 16-Carbon fatty acids (16:0 and 16:1) were synthesized only by the fungi in the mycorrhized roots. Our data strongly suggest that the fatty acid synthase activity of arbuscular-mycorrhizal fungi is expressed exclusively in the intraradical mycelium and indicate that fatty acid metabolism may play a major role in the obligate biotrophism of arbuscular-mycorrhizal fungi. |
| Databáze: | OpenAIRE |
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