Extraocular Motor System Exhibits a Higher Expression of Neurotrophins When Compared with Other Brainstem Motor Systems
| Autor: | Angel M. Pastor, Beatriz Benítez-Temiño, Rosendo G. Hernández, Rosa R. de la Cruz, Sara Morcuende, Silvia Silva-Hucha |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Fisiología, Ministerio de Economía y Competitividad (MINECO). España |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
amyotrophic lateral sclerosis genetic structures Neurotrophin-3 Extraocular muscles nerve growth factor cranial muscles lcsh:RC321-571 03 medical and health sciences 0302 clinical medicine neurotrophin-3 Neurotrophic factors medicine Neuropil brain-derived hypoglossal Amyotrophic lateral sclerosis neurotrophic factor lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Original Research extraocular motoneurons biology musculoskeletal neural and ocular physiology General Neuroscience brain-derived neurotrophic factor Anatomy medicine.disease eye diseases 030104 developmental biology medicine.anatomical_structure Nerve growth factor nervous system biology.protein sense organs Brainstem Neuroscience facial 030217 neurology & neurosurgery Neurotrophin |
| Zdroj: | idUS. Depósito de Investigación de la Universidad de Sevilla instname Frontiers in Neuroscience, Vol 11 (2017) Frontiers in Neuroscience |
| ISSN: | 1662-453X |
| DOI: | 10.3389/fnins.2017.00399 |
| Popis: | Extraocular motoneurons resist degeneration in diseases such as amyotrophic lateral sclerosis. The main objective of the present work was to characterize the presence of neurotrophins in extraocular motoneurons and muscles of the adult rat. We also compared these results with those obtained from other cranial motor systems, such as facial and hypoglossal, which indeed suffer neurodegeneration. Immunocytochemical analysis was used to describe the expression of nerve growth factor, brain-derived neurotrophic factor and neurotrophin-3 in oculomotor, trochlear, abducens, facial, and hypoglossal nuclei of adult rats, and Western blots were used to describe the presence of neurotrophins in extraocular, facial (buccinator), and tongue muscles, which are innervated by the above-mentioned motoneurons. In brainstem samples, brain-derived neurotrophic factor was present both in extraocular and facial motoneuron somata, and to a lesser degree, in hypoglossal motoneurons. Neurotrophin-3 was present in extraocular motor nuclei, while facial and hypoglossal motoneurons were almost devoid of this protein. Finally, nerve growth factor was not present in the soma of any group of motoneurons, although it was present in dendrites of motoneurons located in the neuropil. Neuropil optical density levels were higher in extraocular motoneuron nuclei when compared with facial and hypoglossal nuclei. Neurotrophins could be originated in target muscles, since Western blot analyses revealed the presence of the three molecules in all sampled muscles, to a larger extent in extraocular muscles when compared with facial and tongue muscles. We suggest that the different neurotrophin availability could be related to the particular resistance of extraocular motoneurons to neurodegeneration. MINECO BFU2012-33975 MINECO BFU2015-64515-P. |
| Databáze: | OpenAIRE |
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