Opposite regulation of piRNAs, rRNAs and miRNAs in the blood after subarachnoid hemorrhage
| Autor: | Agnieszka Slowik, Rafał Morga, Slawomir Golda, Dzesika Hoinkis, Joanna Pera, Marek Moskała, Malgorzata Borczyk, Tomasz Dziedzic, Marcin Piechota, Michal Korostynski |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Gene Expression Peripheral blood Context (language use) Biology Small noncoding RNAs 03 medical and health sciences 0302 clinical medicine Drug Discovery microRNA Humans Small nucleolar RNA RNA Small Interfering Gene Genetics (clinical) 030304 developmental biology Genetics 0303 health sciences Retinoid X receptor alpha RNA RNA sequencing Middle Aged Subarachnoid Hemorrhage Intracranial aneurysm DNA binding site MicroRNAs Gene Expression Regulation RNA Ribosomal Molecular Medicine Female Original Article Disease Susceptibility Estrogen receptor alpha Cell-Free Nucleic Acids 030217 neurology & neurosurgery Biomarkers Transcription Factors |
| Zdroj: | Journal of Molecular Medicine (Berlin, Germany) |
| Popis: | Multiple classes of small RNAs (sRNAs) are expressed in the blood and are involved in the regulation of pivotal cellular processes. We aimed to elucidate the expression patterns and functional roles of sRNAs in the systemic response to intracranial aneurysm (IA) rupture. We used next-generation sequencing to analyze the expression of sRNAs in patients in the acute phase of IA rupture (first 72 h), in the chronic phase (3–15 months), and controls. The patterns of alterations in sRNA expression were analyzed in the context of clinically relevant information regarding the biological consequences of IA rupture. We identified 542 differentially expressed sRNAs (108 piRNAs, 99 rRNAs, 90 miRNAs, 43 scRNAs, 36 tRNAs, and 32 snoRNAs) among the studied groups with notable differences in upregulated and downregulated sRNAs between the groups and sRNAs categories. piRNAs and rRNAs showed a substantial decrease in RNA abundance that was sustained after IA rupture, whereas miRNAs were largely upregulated. Downregulated sRNA genes included piR-31080, piR-57947, 5S rRNA, LSU-rRNA, and SSU-rRNA s. Remarkable enrichment in the representation of transcription factor binding sites was revealed in genomic locations of the regulated sRNA. We found strong overrepresentation of glucocorticoid receptor, retinoid x receptor alpha, and estrogen receptor alpha binding sites at the locations of downregulated piRNAs, tRNAs, and rRNAs. This report, although preliminary and largely proof-of-concept, is the first to describe alterations in sRNAs abundance levels in response to IA rupture in humans. The obtained results indicate novel mechanisms that may constitute another level of control of the inflammatory response.Key messagesA total of 542 sRNAs were differentially expressed after aneurysmal SAH comparing with controlspiRNAs and rRNAs were upregulated and miRNAs were downregulated after IA ruptureThe regulated sRNA showed an enrichment in the representation of some transcription factor binding sitespiRNAs, tRNAs, and rRNAs showed an overrepresentation for GR, RXRA, and ERALPHA binding sites |
| Databáze: | OpenAIRE |
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