Isolation and characterization of microsatellite loci in Astroniumgraveolens (Anacardiaceae) and cross amplification in related species
| Autor: | E. Jacob Cristóbal-Perez, Nick Harvey, Mauricio Quesada, Karman F. Sánchez-Gómez |
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| Rok vydání: | 2020 |
| Předmět: |
Gene Flow
Genetic Markers 0301 basic medicine Conservation genetics Heterozygote DNA Plant Genotype Anacardiaceae Population Locus (genetics) Biology Linkage Disequilibrium Trees Gene flow 03 medical and health sciences 0302 clinical medicine Gene Frequency Species Specificity Genetics Allele education Mexico Molecular Biology Alleles DNA Primers Genomic Library education.field_of_study Polymorphism Genetic General Medicine 030104 developmental biology Genetic Loci Evolutionary biology Genetic marker 030220 oncology & carcinogenesis Genetic structure Microsatellite Microsatellite Repeats |
| Zdroj: | Molecular Biology Reports. 47:4003-4007 |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | Microsatellite markers are a useful genetic tool to answer ecological and conservation genetics questions. Microsatellite primers were developed and characterized to evaluate forest fragmentation effects on genetic structure, diversity and gene flow patterns in the dioecious tropical tree Astroniumgraveolens (Anacardiaceae). Using genomic library enrichment, sixteen microsatellite loci were developed for A.graveolens. Polymorphism was evaluated in 80 individuals from four localities in Mexico. The overall number of alleles detected was 63, average alleles per locus 3.9 with a range from one to 11 per locus. Cross amplification trails on related species in the Anacardiaceae family: Spondiaspurpurea and Amphipterygiumadstringens, achieved successful amplification for all primers. Microsatellite markers described here are the first to be characterized for A.graveolens. These genetic markers will be a useful tool to assess the genetic consequences of habitat fragmentation and selective logging on A.graveolens populations. Cross amplification success on S.purpureaandA.adstringens suggest that they may be used for population genetic studies in other species within the family. |
| Databáze: | OpenAIRE |
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