Discovery of a novel DNA binding agent via design and synthesis of new thiazole hybrids and fused 1,2,4-triazines as potential antitumor agents: Computational, spectrometric and in silico studies
| Autor: | K. A. Ismail, Amira F. El-Yazbi, Ibrahim M. Labouta, Marwa H. El-Wakil, Hayam M. A. Ashour, Mounir A. Khalil |
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| Rok vydání: | 2019 |
| Předmět: |
DNA damage
In silico Antineoplastic Agents Apoptosis 01 natural sciences Biochemistry Structure-Activity Relationship chemistry.chemical_compound Neoplasms Drug Discovery Tumor Cells Cultured Humans Thiazole Molecular Biology Cell Proliferation Molecular Structure Triazines 010405 organic chemistry Organic Chemistry Hyperchromicity DNA Druglikeness In vitro 0104 chemical sciences Thiazoles 010404 medicinal & biomolecular chemistry chemistry Drug Design Drug Screening Assays Antitumor DNA Binding Agent |
| Zdroj: | Bioorganic Chemistry. 90:103089 |
| ISSN: | 0045-2068 |
| Popis: | New series of furan–thiazole hybrids (3a-f), thiazolo[2,3-c]-1,2,4-triazines (4a-f), their bioisosteres 1,3,4-thiadiazolo[2,3-c]-1,2,4-triazines (8a-d) and 1,2,4-triazino[4,3-b]-1,2,4-triazines (13a-e) were designed, synthesized and evaluated for their in vitro antitumor activities at the National Cancer Institute (NCI, USA). Among the synthesized compounds, 3d was found to exhibit promising broad spectrum antitumor activity (GI50 MG-MID = 14.22 µM) in a five-dose assay against the full panel NCI-cancer cell lines. 3d displayed higher antitumor activity against most tested cancer cell lines than 5-FU as reference. COMPARE analysis and molecular electrostatic potential computational study revealed that 3d probably exerts its antitumor properties through DNA binding similar to Clomesone. Further DNA binding studies using fluorescent terbium (Tb+3) probe revealed increased fluroresence of DNA-3d-Tb+3 mixture due to damage of the double-stranded DNA. Also, UV–vis absorption study was conducted which showed hyperchromic shift in DNA absorption confirming 3d-induced DNA damage. The assessed potency of 3d-induced DNA damage of calf thymus DNA showed a concentration as low as 2.04 ng/mL for a detectable DNA damage. Moreover, in silico calculation of physicochemical properties and druglikeness were in compliance to Lipinski’s rule. |
| Databáze: | OpenAIRE |
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