Cortactin Is a Functional Target of E-cadherin-activated Src Family Kinases in MCF7 Epithelial Monolayers
| Autor: | Suzie Verma, Alpha S. Yap, Robert W. McLachlan, Gang Ren, Scott A. Weed, Falak Helwani |
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| Rok vydání: | 2009 |
| Předmět: |
Cell signaling
Cell Communication macromolecular substances Models Biological Biochemistry chemistry.chemical_compound Dogs Molecular Basis of Cell and Developmental Biology Cell Line Tumor Cell Adhesion Animals Humans Phosphorylation Cell adhesion Cytoskeleton Molecular Biology biology Cadherin Epithelial Cells Tyrosine phosphorylation Cell Biology Cadherins Actin cytoskeleton Actins Cell biology src-Family Kinases chemistry biology.protein Tyrosine Cortactin |
| Zdroj: | Journal of Biological Chemistry. 284:18913-18922 |
| ISSN: | 0021-9258 |
| Popis: | Src family kinases (SFKs) signal in response to E-cadherin to support cadherin adhesion and the integrity of cell-cell contacts (McLachlan, R. W., Kraemer, A., Helwani, F. M., Kovacs, E. M., and Yap, A. S. (2007) Mol. Biol. Cell 18, 3214–3223). We now identify the actin-regulatory protein, cortactin, as a target of E-cadherin-activated SFK signaling. Tyr-phosphorylated cortactin was found at cell-cell contacts in established epithelial monolayers, and cortactin became acutely tyrosine-phosphorylated when E-cadherin adhesion was engaged. In all circumstances, cortactin tyrosine phosphorylation was blocked by inhibiting SFK signaling. Importantly, Tyr-phosphorylated cortactin was necessary to preserve the integrity of cadherin contacts and the perijunctional actin cytoskeleton. Moreover, expression of a phosphomimetic cortactin mutant could prevent SFK blockade from disrupting cadherin organization, thereby placing cortactin functionally downstream of SFK signaling at cadherin adhesions. We conclude that SFK and cortactin constitute an important signaling pathway that functionally links E-cadherin adhesion and the actin cytoskeleton. |
| Databáze: | OpenAIRE |
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