Differential osteopontin expression in human osteoblasts derived from iliac crest and alveolar bone and its role in early stages of angiogenesis
| Autor: | Brigitte Altmann, Tobias Fretwurst, Diana Huelter-Hassler, Susanne Nahles, Katja Nelson, Guenter Finkenzeller, Martin Wein, Thorsten Steinberg |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Angiogenesis Endocrinology Diabetes and Metabolism Neovascularization Physiologic Iliac crest Umbilical vein Ilium 03 medical and health sciences 0302 clinical medicine Endocrinology stomatognathic system Cell Movement Gene expression medicine Alveolar Process Human Umbilical Vein Endothelial Cells Animals Humans Orthopedics and Sports Medicine Osteopontin RNA Messenger Dental alveolus Cell Proliferation Tube formation Osteoblasts biology General Medicine 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation 030220 oncology & carcinogenesis Cancer research biology.protein Female Function (biology) |
| Zdroj: | Journal of bone and mineral metabolism. 37(1) |
| ISSN: | 1435-5604 |
| Popis: | In our previous study, we revealed significant differences of osteopontin (OPN) gene expression in primary human osteoblasts (HOBs) derived from iliac crest bone (iHOBs) and alveolar bone (aHOBs). The present study aims at assigning this discriminative expression to a possible biologic function. OPN is known to be involved in several pathologic and physiologic processes, among others angiogenesis. Therefore, we studied the reaction of human umbilical vein endothelial cells (HUVECs) to HOB-derived OPN regarding angiogenesis. To this end, human primary explant cultures of both bone entities from ten donors were established. Subsequent transcription analysis detected higher gene expression of OPN in iHOBs compared to aHOBs, thereby confirming the results of our previous study. This difference was particularly apparent when cultures were derived from female donors. Hence, OPN protein expression as well as the angiogenic potential of OPN was analyzed, originating from HOBs of one female donor. In accordance to the gene expression level, secreted OPN was more abundant in the supernatant of iHOBs than in aHOBs. Moreover, secreted OPN was found to stimulate migration of HUVECs, but not proliferation or tube formation. These results indicate an involvement in very early stages of angiogenesis and a functional distinction of OPN from HOBs derived from different bone entities. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink