Outbreak of Opportunistic Ascending Pyelonephritis with Numerous Yeast after Experimental Humanization Surgery in NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ and NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl/SzJ Immunodeficient Mice
| Autor: | Leah M Zadrozny, Barry A. Rosenzweig, Lauren R. Brinster, Kristina E. Howard |
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| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
040301 veterinary sciences Mouse Models Nod Opportunistic Infections General Biochemistry Genetics and Molecular Biology Enterococcus faecalis Disease Outbreaks Rodent Diseases 0403 veterinary science Immunocompromised Host Mice Inbred NOD medicine Animals Humans Candida albicans Pyelonephritis General Veterinary biology business.industry Candidiasis Staphylococcus xylosus Outbreak Tail vein 04 agricultural and veterinary sciences biology.organism_classification Yeast Surgery Tissue Transplantation Female Histopathology business |
| Zdroj: | Comparative Medicine. 68:353-359 |
| ISSN: | 1532-0820 |
| Popis: | Unexpected mortality occurred in a group of 12 NOD.Cg-Prkdc(scid)Il2rg(tm1Wjl)/SzJ (NSG) and 12 NOD.Cg-Rag1(tm1Mom) Il2rg(tm1Wjl)/SzJ (NRG) immunodeficient mice. At 10 d after routine bone marrow–liver–thymus humanization surgery, 9 mice were found dead without observation of initiating clinical signs; 1 d later (day 11), 3 additional mice showed signs of morbidity, including severe hunching, lateral recumbency, slow movement, shallow respiration, and decreased response to external stimulus. All remaining mice rapidly decompensated and were found dead or were euthanized within 4 d after the first death. Histopathology revealed severe ascending pyelonephritis with numerous yeast. Cultures in some mice were positive for Enterococcus faecalis or Staphylococcus xylosus, 2 bacteria considered commensals in rodents. In addition, Candida albicans was cultured from some animals. Further investigation revealed that a restraining device used for tail vein injections was the likely fomite harboring Candida organisms. These findings indicate that ascending pyelonephritis, with Candida as the etiologic agent, can cause significant mortality in NSG and NRG immunodeficient mice. |
| Databáze: | OpenAIRE |
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