Comparative evaluation of the cost and efficiency of four types of sexing methods for the production of dairy female calves
| Autor: | Ryotaro Miura, Kiyoshi Akiyama, Hitoshi Ushijima, Masahiro Osada, Hiroshi Nakagawa, Tomoko Obuchi, Nobutada Sakagami, Masaya Geshi, Takeyuki Ozawa, Michiko Hayashi |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Sex Determination Analysis Pregnancy Rate medicine.medical_treatment Cost-Benefit Analysis Oocyte Retrieval Semen Sexing Fertilization in Vitro Biology Breeding Comparative evaluation Animal science Embryo transfer cost performance Pregnancy X-sorted semen Follicular phase medicine Animals Sex Preselection Insemination Artificial Artificial insemination Embryo Embryo Mammalian Flow Cytometry Spermatozoa Embryo transfer In Vitro Oocyte Maturation Techniques Pregnancy rate Dairying Bovine female embryo Animal Science and Zoology Cattle Female Original Article In vitro embryo production Ovum pick-up |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 |
| Popis: | This study was conducted to evaluate and compare the economic benefits of different embryo sexing methods, based on the cost per female dairy calf produced. Female calves were produced from four kinds of female embryos: (1) those collected from superstimulated donors at 7-8 days after artificial insemination (AI) with X-sorted semen; (2) those sex-determined by loop-mediated isothermal amplification assay of a biopsy sample of embryos collected from superstimulated donors after AI with conventional unsorted semen; (3) those obtained by invitro embryo production (IVEP), using X-sorted semen and in vitro-matured oocytes collected from donors by ovum pick-up (OPU); and (4) those obtained by IVEP, using X-sorted semen and oocytes collected by OPU after dominant follicle ablation and follicle growth stimulation of the donors. The respective productivities of female calves per technical service and the total production cost per female calf of each sexing method were compared. The production cost per female calf (66,537 JPY), as calculated from the number of female calves per service (1.30), pregnancy rate of transfer (42.9%), rate of female calves obtained (92.9%), and total cost of the method (56,643 JPY plus embryo transfer fee), was less for IVEP with X-sorted semen and follicular growth-stimulated (FGS) oocytes than for the other groups (P < 0.05). The results demonstrate that embryo production with X-sorted semen and FGS oocytes provides a more efficient method for producing female calves than the other embryo sexing methods. |
| Databáze: | OpenAIRE |
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