Analysis of a Microbial Community Oxidizing Inorganic Sulfide and Mercaptans
| Autor: | Kathleen E. Duncan, Robert R. Beitle, Patricia A. Rider, Kerry L. Sublette, Ravi Kolhatkar, Julie A. Conner, Anita Stepp |
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| Rok vydání: | 2001 |
| Předmět: |
Electrophoresis
Powdered activated carbon treatment Microorganism Sulfides Enrichment culture Microbiology Industrial Microbiology Bioreactors RNA Ribosomal 16S Bioreactor Sulfhydryl Compounds Phospholipids Microscopy Chromatography Bacteria biology Fatty Acids Sequence Analysis DNA Cells Immobilized biology.organism_classification Aerobiosis Activated sludge Proteobacteria Energy source Oxidation-Reduction Temperature gradient gel electrophoresis Biotechnology |
| Zdroj: | Biotechnology Progress. 17:768-774 |
| ISSN: | 8756-7938 |
| DOI: | 10.1021/bp0100530 |
| Popis: | Successful treatment of refinery spent-sulfidic caustic (which results from the addition of sodium hydroxide solutions to petroleum refinery waste streams) was achieved in a bioreactor containing an enrichment culture immobilized in organic polymer beads with embedded powdered activated carbon (Bio-Sep). The aerobic enrichment culture had previously been selected using a gas mixture of hydrogen sulfide and methyl mercaptan (MeSH) as the sole carbon and energy sources. The starting cultures for the enrichment consisted of several different Thiobacilli spp. (T. thioparus, T. denitrificans, T. thiooxidans, and T. neopolitanus), as well as activated sludge from a refinery aerobic wastewater treatment system and sludge from an industrial anaerobic digester. Microscopic examination (light and SEM) of the beads and of microbial growth on the walls of the bioreactor revealed a great diversity of microorganisms. Further characterization was undertaken starting with culturable aerobic heterotrophic microorganisms (sequencing of PCR-amplified DNA coding for 16S rRNA, Gram staining) and by PCR amplification of DNA coding for 16S rRNA extracted directly from the cell mass, followed by the separation of the PCR products by DGGE (denaturing gradient gel electrophoresis). Eight prominent bands from the DGGE gel were sequenced and found to be closest to sequences of uncultured Cytophagales (3 bands), Gram-positive cocci (Micrococcineae), alpha proteobacteria (3 bands), and an unidentified beta proteobacterium. Culturable microbes included several genera of fungi as well as various Gram-positive and Gram-negative heterotrophic bacteria not seen in techniques using direct DNA extraction. |
| Databáze: | OpenAIRE |
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