Visualization of extracellular DNA released during border cell separation from the root cap
| Autor: | Gilberto Curlango-Rivera, David A. Huskey, Martha C. Hawes, Zhongguo Xiong, Fushi Wen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Extracellular Traps DNA Plant Cell Meristem Plant Science Plant Roots Zea mays 03 medical and health sciences chemistry.chemical_compound Botany Border cells Genetics medicine Extracellular Centrifugation DAPI Root cap Ecology Evolution Behavior and Systematics biology Peas biology.organism_classification Cell biology 030104 developmental biology medicine.anatomical_structure chemistry DNA |
| Zdroj: | American journal of botany. 104(7) |
| ISSN: | 1537-2197 |
| Popis: | Premise of the study Root border cells are programmed to separate from the root cap as it penetrates the soil environment, where the cells actively secrete >100 extracellular proteins into the surrounding mucilage. The detached cells function in defense of the root tip by an extracellular trapping process that also requires DNA, as in mammalian white blood cells. Trapping in animals and plants is reversed by treatment with DNase, which results in increased infection. The goal of this study was to evaluate the role of DNA in the structural integrity of extracellular structures released as border cells disperse from the root tip upon contact with water. Methods DNA stains including crystal violet, toluidine blue, Hoechst 33342, DAPI, and SYTOX green were added to root tips to visualize the extracellular mucilage as it absorbed water and border cell populations dispersed. DNase I was used to assess structural changes occurring when extracellular DNA was degraded. Key results Complex masses associated with living border cells were immediately evident in response to each stain, including those that are specific for DNA. Treating with DNase I dramatically altered the appearance of the extracellular structures and their association with border cells. No extracellular DNA was found in association with border cells killed by freezing or high-speed centrifugation. This observation is consistent with the hypothesis that, as with border cell extracellular proteins, DNA is secreted by living cells. Conclusion DNA is an integral component of border cell extracellular traps. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text