Loci controlling lymphocyte production of interferon gamma after alloantigen stimulation in vitro and their co-localization with genes controlling lymphocyte infiltration of tumors and tumor susceptibility

Autor: Peter Demant, Yahya Sohrabi, Jana Badalová, Lei Quan, Alphons P. M. Stassen, Helena Havelková, Magdalena Krulova, Marie Lipoldová, Jarmila Vojtíšková
Přispěvatelé: Genetica & Celbiologie, RS: GROW - School for Oncology and Reproduction
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Male
Isoantigens
Cancer Research
Lymphocyte
medicine.medical_treatment
Immunology
Tumor susceptibility
Stimulation
Biology
Genetic control of interferon gamma production
Interferon-gamma
Mice
03 medical and health sciences
Lymphocytes
Tumor-Infiltrating
0302 clinical medicine
Lymphocyte infiltration of tumors
medicine
Splenocyte
Animals
Immunology and Allergy
Interferon gamma
Gene mapping
030304 developmental biology
Mice
Inbred BALB C
0303 health sciences
Chromosome Mapping
Cancer
Immunotherapy
medicine.disease
Molecular biology
Mice
Mutant Strains
In vitro
3. Good health
Mice
Inbred C57BL
medicine.anatomical_structure
Genetic control of interferon γ production
Oncology
Genetic Loci
030220 oncology & carcinogenesis
Female
Original Article
Lymphocyte Culture Test
Mixed
Infiltration (medical)
medicine.drug
Zdroj: Cancer Immunology Immunotherapy, 59(2), 203-213. Springer
Cancer Immunology, Immunotherapy
ISSN: 0340-7004
Popis: Low infiltration of lymphocytes into cancers is associated with poor prognosis, but the reasons why some patients exhibit a low and others a high infiltration of tumors are unknown. Previously we mapped four loci (Lynf1-Lynf4) controlling lymphocyte infiltration of mouse lung tumors. These loci do not encode any of the molecules that are involved in traffic of lymphocytes. Here we report a genetic relationship between these loci and the control of production of IFN gamma in allogeneic mixed lymphocyte cultures (MLC). We found that IFN gamma production by lymphocytes of O20/A mice is lower than by lymphocytes of OcB-9/Dem mice (both H2 (pz) ) stimulated in MLC by irradiated splenocytes of C57BL/10SnPh (H2 (b) ) or BALB/cHeA (H2 (d) ) mice, or by ConA. IFN gamma production in MLCs of individual (O20 x OcB-9)F(2) mice stimulated by irradiated C57BL/10 splenocytes and genotyped for microsatellite markers revealed four IFN gamma-controlling loci (Cypr4-Cypr7), each of which is closely linked with one of the four Lynf loci and with a cluster of susceptibility genes for different tumors. This suggests that inherited differences in certain lymphocyte responses may modify their propensity to infiltrate tumors and their capacity to affect tumor growth.
Databáze: OpenAIRE
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