Arginine‐ but not alanine‐rich carboxy‐termini trigger nuclear translocation of mutant keratin 10 in ichthyosis with confetti

Autor: Andreas Volz, Hedwig Wariwoda, I. Spoerri, Peter Itin, Bettina Burger, M. Aushev, Julia Reichelt, E. Imahorn, Oliver Patrick March, Patricia Renz, Sarah Von Arb
Rok vydání: 2019
Předmět:
Keratinocytes
0301 basic medicine
keratin 10
Green Fluorescent Proteins
Active Transport
Cell Nucleus
Arginine
Cell Line
Frameshift mutation
03 medical and health sciences
carboxy terminus
0302 clinical medicine
Mutant protein
Keratin
medicine
Humans
arginine‐rich C‐terminus
nuclear localization
Frameshift Mutation
Cellular localization
Cell Nucleus
chemistry.chemical_classification
Alanine
Microscopy
Confocal
integumentary system
Chemistry
Original Articles
Exons
Cell Biology
Ichthyosiform Erythroderma
Congenital
Keratin-10
medicine.disease
Keratin 1
Subcellular localization
Cell biology
ichthyosis with confetti
030104 developmental biology
alanine‐rich C‐terminus
030220 oncology & carcinogenesis
KRT10
Mutation
Molecular Medicine
Original Article
Ichthyosis with confetti
Nuclear localization sequence
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
1582-1838
DOI: 10.1111/jcmm.14727
Popis: Ichthyosis with confetti (IWC) is a genodermatosis associated with dominant‐negative variants in keratin 10 (KRT10) or keratin 1 (KRT1). These frameshift variants result in extended aberrant proteins, localized to the nucleus rather than the cytoplasm. This mislocalization is thought to occur as a result of the altered carboxy (C)‐terminus, from poly‐glycine to either a poly‐arginine or ‐alanine tail. Previous studies on the type of C‐terminus and subcellular localization of the respective mutant protein are divergent. In order to fully elucidate the pathomechanism of IWC, a greater understanding is critical. This study aimed to establish the consequences for localization and intermediate filament formation of altered keratin 10 (K10) C‐termini. To achieve this, plasmids expressing distinct KRT10 variants were generated. Sequences encoded all possible reading frames of the K10 C‐terminus as well as a nonsense variant. A keratinocyte line was transfected with these plasmids. Additionally, gene editing was utilized to introduce frameshift variants in exon 6 and exon 7 at the endogenous KRT10 locus. Cellular localization of aberrant K10 was observed via immunofluorescence using various antibodies. In each setting, immunofluorescence analysis demonstrated aberrant nuclear localization of K10 featuring an arginine‐rich C‐terminus. However, this was not observed with K10 featuring an alanine‐rich C‐terminus. Instead, the protein displayed cytoplasmic localization, consistent with wild‐type and truncated forms of K10. This study demonstrates that, of the various 3′ frameshift variants of KRT10, exclusively arginine‐rich C‐termini lead to nuclear localization of K10.
Databáze: OpenAIRE
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