Subspecies variation in Greek strains of Chlamydophila abortus
| Autor: | Victoria I. Siarkou, Alexandros Kotsis, Alexandros Lambropoulos, Sofia Chrisafi, O. Papadopoulos |
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| Rok vydání: | 2002 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Restriction Mapping Sheep Diseases DNA Ribosomal Polymerase Chain Reaction Microbiology law.invention law RNA Ribosomal 16S DNA Ribosomal Spacer Animals Chlamydophila Infections Gene Phylogeny Polymerase chain reaction Chlamydia psittaci Genetics Goat Diseases Sheep Base Sequence Greece General Veterinary biology Chlamydophila Goats Gene Amplification Genetic Variation General Medicine Abortion Veterinary Ribosomal RNA biology.organism_classification Molecular biology Chlamydophila abortus RNA Ribosomal 23S Restriction enzyme Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Veterinary Microbiology. 85:145-157 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/s0378-1135(01)00506-5 |
| Popis: | The Greek chlamydial strains FAS, FAG, VPG and LLG, isolated from aborted sheep or goat foetuses, had been previously characterized as divergent on the basis of mouse cross-protection experiments, with LLG and its homologous POS significantly different from the rest in inclusion morphology, polypeptide profiles and reactivity with monoclonal antibodies. To determine the genetic basis of their divergence the 16S-23S ribosomal intergenic spacer was analysed by RFLP analysis of PCR 16SF2/23R amplicons. Using the restriction enzymes BfaI, SfcI, HpaI, BclI, DdeI and AclI, the strains were classified as Chlamydophila abortus. However, digestion with RsaI made it possible to differentiate strains FAS, FAG and VPG from strains LLG and POS, generating DNA fragments of 530/55 and 585bp, respectively. By subsequent sequence analysis of the 23S domain I rRNA gene only strain FAS was identical to reference strain A22 of C. abortus. Strains FAG and VPG presented an identical nucleotide deviation at position 593 of signature sequences. Strains LLG and POS presented three identical nucleotide deviations at positions 156, 186 and 307. Variation within the domain I signature sequences for the examined abortion strains was < or =0.69%. In conclusion, substantial genetic and biological diversity among strains of C. abortus was demonstrated, suggesting that subspecies variation status for certain strains may be applicable. Our findings suggest that differentiation may be possible at a subspecies level by RFLP analysis. |
| Databáze: | OpenAIRE |
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