Improved sample dispersion in cryo-EM using 'perpetually-hydrated' graphene oxide flakes
| Autor: | Masao Yamashita, Cathal Cassidy, Tsumoru Shintake, Seita Taba, Kun-Lung Li, Martin Cheung, Hidehito Adaniya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Cryo-electron microscopy Graphene Cryoelectron Microscopy Oxide 02 engineering and technology Electron 021001 nanoscience & nanotechnology law.invention Specimen Handling 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Chemical engineering Structural Biology law Microscopy Particle Sample preparation Graphite 0210 nano-technology Dispersion (chemistry) |
| Zdroj: | Journal of Structural Biology. 204(1):75-79 |
| ISSN: | 1047-8477 |
| Popis: | For many macromolecular complexes, the inability to uniformly disperse solubilized specimen particles within vitreous ice films precludes their analysis by cryo-electron microscopy (cryo-EM). Here, we introduce a sample preparation process using "perpetually-hydrated" graphene oxide flakes as particle support films, and report vastly improved specimen dispersion. Furthermore, we provide evidence that the presence of graphene oxide flakes in vitreous ice results in a significant reduction in electron beam-induced specimen decomposition. The new method introduced in this study incorporates hydrated graphene oxide flakes into a standard sample preparation regime, without the need for additional tools or devices, making it a cost-effective and easily adoptable alternative to currently available sample preparation approaches. |
| Databáze: | OpenAIRE |
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