Decreased aortic smooth muscle contraction in a rat model of multibacterial sepsis
| Autor: | Philippe Giummelly, Arnaud Mansard, Jeffrey Atkinson, Changhua Wang |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Contraction (grammar) Receptors Cytoplasmic and Nuclear Vasodilation In Vitro Techniques Muscle Smooth Vascular Contractility Soluble Guanylyl Cyclase Quinoxalines Sepsis Internal medicine medicine Animals Pharmacology (medical) Enzyme Inhibitors Rats Wistar Aorta Pharmacology Oxadiazoles biology business.industry Endothelial Cells Smooth muscle contraction Rats Nitric oxide synthase Disease Models Animal NG-Nitroarginine Methyl Ester Endocrinology Guanylate Cyclase Anesthesia cardiovascular system biology.protein Nitric Oxide Synthase medicine.symptom business Soluble guanylyl cyclase Vasoconstriction Muscle Contraction Muscle contraction |
| Zdroj: | Fundamental and Clinical Pharmacology. 18:679-683 |
| ISSN: | 1472-8206 0767-3981 |
| DOI: | 10.1111/j.1472-8206.2004.00293.x |
| Popis: | We investigated whether blockade of the smooth muscle cell (SMC) inducible nitric oxide synthase (iNOS)-soluble guanylyl cyclase (sGC) vasodilator pathway would restore the fall in vasoreactivity produced by sepsis following cecal ligation and perforation (CLP) in rats. Contraction of adjacent aortic rings paired for the presence or absence of endothelial cells (EC) was recorded following high [K(+)](e) (40 mm) or norepinephrine (NE, 10(-8) to 10(-5) m) in the presence of the nitric oxide synthase inhibitor (NOS), N(G)-nitro-l-arginine methyl ester (l-NAME, 0.3 mm) or the sGC inhibitor, 1H-[1,2,4]oxadiazolo[4,3-alpha]quinoxalin-1-one (ODQ, 5 mum). In EC-denuded rings, sepsis halved SMC contraction induced by high [K(+)](e) or NE; neither l-NAME nor ODQ produced an increase in NE E(max) or high [K(+)](e)-evoked contraction. In conclusion, SMC contractility is globally reduced in CLP; this reduction does not appear to be explained by induction of SMC NOS in this CLP model. |
| Databáze: | OpenAIRE |
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