Fluorescent Peptoids as Selective Live Cell Imaging Probes
| Autor: | José R. Corrêa, Samira L. M. Soares, Saulo T. A. Passos, Wender A. Silva, Brenno A. D. Neto |
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| Rok vydání: | 2016 |
| Předmět: |
Peptidomimetic
media_common.quotation_subject Mitochondrion 010402 general chemistry 01 natural sciences Peptoids Live cell imaging Cell Line Tumor Organelle Biomarkers Tumor Humans Internalization Volume concentration Fluorescent Dyes media_common 010405 organic chemistry Chemistry Organic Chemistry Molecular biology Fluorescence Mitochondria 0104 chemical sciences Microscopy Fluorescence Biophysics Peptidomimetics Breast cancer cells |
| Zdroj: | The Journal of Organic Chemistry. 81:2646-2651 |
| ISSN: | 1520-6904 0022-3263 |
| Popis: | This paper describes the synthesis of fluorescent peptoids using the Ugi multicomponent reaction (4CR). The four synthesized structures had their photophysical properties evaluated and their potential as biomarkers established. The peptidomimetics were used at very low concentrations (10 nM) to follow their internalization in breast cancer cells and had their localization precisely determined. One of the new peptoids displayed mitochondrial affinity and stained this important organelle selectively. Co-staining experiments using MitoTracker Red confirmed the localization inside live cells. |
| Databáze: | OpenAIRE |
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