Rapid autographic method for detection of enzymatic browning inhibitors based on enzyme immobilization
| Autor: | Abel Eduardo Farroni, Oscar B. Micheloni, Paula García, Ricardo L. E. Furlan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Immobilized enzyme
POLYPHENOL OXIDASE Hexylresorcinol ENZYMATIC BROWNING Ascorbic Acid 01 natural sciences Polyphenol oxidase Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Browning Enzyme Inhibitors Catechol oxidase chemistry.chemical_classification Chromatography biology Chemistry Otras Ciencias Naturales y Exactas 010401 analytical chemistry 04 agricultural and veterinary sciences General Medicine Ascorbic acid 040401 food science Maillard Reaction 0104 chemical sciences AUTOGRAPHY Enzyme Polyphenol Malus NATURAL EXTRACTS biology.protein Kojic acid Catechol Oxidase CIENCIAS NATURALES Y EXACTAS Food Science |
| Zdroj: | Food Chemistry. 269:638-643 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2018.07.025 |
| Popis: | This work describes a TLC-coupled bioautographic assay suitable for the separation and detection of apple polyphenol oxidase (PPO) inhibitors from natural extracts. PPO was immobilised in agar containing L-DOPA as substrate and 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) to enhance colour development. The inhibition was detected as white spots on reddish background. Minimum amount of PPO inhibitors detected was 0.0125 µg of 4-hexylresorcinol, 0.025 µg of ascorbic acid, 0.5 µg of cysteine and 1 µg of kojic acid. The assay was compatible with normal and reverse phase TLC systems and allows detecting compounds that directly had action on the enzyme as well as agents that could convert quinones back to their reduced form. The chromatographic run evidenced the different nature of enzymatic browning inhibitory compounds from garlic and onion extracts. Using natural enzymes will provide a fast and cheap alternative for target specific exploration of natural enzymatic inhibitors. Fil: Micheloni, Oscar Bernardo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina Fil: Farroni, Abel Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: García, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina Fil: Furlan, Ricardo Luis Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario. Universidad Nacional de Rosario. Instituto de Investigaciones para el Descubrimiento de Fármacos de Rosario; Argentina |
| Databáze: | OpenAIRE |
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