Possible Neuroprotective Effects of Magnesium Sulfate and Melatonin as Both Pre- and Post-Treatment in a Neonatal Hypoxic-Ischemic Rat Model

Autor: Tulin Alkan, Merih Cetinkaya, Nilgün Köksal, Ilker Mustafa Kafa, Mustafa Ayberk Kurt, Fadil Ozyener
Přispěvatelé: Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Saǧlıǧı ve Hastalıkları Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Psikoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Anatomi Anabilim Dalı., Çetinkaya, Merih, Alkan, Tülin, Özyener, Fadil, Kafa, İlker Mustafa, Kurt, Mustafa Ayberk, Köksal, Nilgün, AAH-1792-2021, AAG-7125-2021, AAH-1641-2021, AAG-8393-2021, AAR-4341-2020
Rok vydání: 2010
Předmět:
genetic structures
Drug Evaluation
Preclinical
Apoptosis
Hippocampal CA3 region
Pharmacology
Pediatrics
Hypoxia ischemia
Animal tissue
Rats
Sprague-Dawley
Hippocampal CA1 region
Pregnancy
Brain-injury
Hypoxia-ischemia
Pre and post
Priority journal
Melatonin
Conference paper
Magnesium
Neuroprotection
Drug Combinations
Damage
Neuroprotective Agents
Randomized controlled trial
Anesthesia
Hypoxia-Ischemia
Brain
Newborn rats
Female
Protects
Infants
Hypoxic ischemic encephalopathy
Pretreatment
Eclampsia
Magnesium Sulfate
Pregnancy Toxemia
Algorithms
medicine.drug
Sodium chloride
Rat model
chemistry.chemical_element
Drug Administration Schedule
Brain ischemia
medicine
Brain infarction size
Animals
Animal model
Dentate gyrus
Animal experiment
Brain hypoxia
Infusion
Consequences
Hypoxic ischemic
Rattus
business.industry
Birth asphyxia
Adrenal cortex
Nonhuman
Rats
Disease Models
Animal
Animals
Newborn
chemistry
Pediatrics
Perinatology and Child Health
Rat
Cell-death
business
Controlled study
Developmental Biology
Zdroj: Neonatology. 99:302-310
ISSN: 1661-7819
1661-7800
DOI: 10.1159/000320643
Popis: Background: Perinatal hypoxia-ischemia is a major cause of mortality and long-term neurological deficits. Objectives: The objective of this study was to compare the effects of two neuroprotective agents; magnesium sulfate and melatonin, administered alone or in combination, on brain infarct volume and TUNEL positivity in a neonatal hypoxic-ischemic (HI) rat model. Methods: After being anesthetized, 7-day-old pups (n = 80) underwent ischemia followed by exposure to hypoxia for 2 h. The pups were then divided equally and randomly into 4 groups in order to receive the vehicle (saline, control group), magnesium sulfate, melatonin or a combination of magnesium sulfate and melatonin. Treatments were administered intraperitoneally three times; the first being just before ischemia, the second after hypoxia and the third 24 h after the second dose. The pups were sacrificed on postnatal day 10, their brains harvested and evaluated for infarct volume and neuronal apoptosis. Results: Percent infarcted brain volume was significantly reduced in pups receiving the drugs (either magnesium sulfate, melatonin or their combination) compared with those receiving the vehicle. In addition, TUNEL staining showed markedly reduced numbers of TUNEL-positive cells per unit area in the CA1, CA3 and dentate gyrus regions of the hippocampus and in the cortex. However, no statistically significant differences were found regarding percent infarcted brain volume and number of TUNEL-positive cells among the drug-treated groups. Conclusions: Magnesium sulfate and melatonin, two agents acting at different stages of HI brain damage, administered either alone or in combination, significantly reduced the percent infarcted brain volume and TUNEL positivity, suggesting that these agents may confer a possible benefit in the treatment of infants with HI encephalopathy.
Databáze: OpenAIRE
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