Susceptibility of isogeneic ginbuna Carassius auratus langsdorfii Temminck et Schlegel to cyprinid herpesvirus-2 (CyHV-2) as a model species
| Autor: | A. Nanjo, K. Yoshii, Goshi Kato, Takashi Sakamoto, Teruyuki Nakanishi, M. Tanaka, M Saito, Motohiko Sano, Hideo Fukuda, T. Shibata |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Necrosis Hot Temperature Veterinary (miscellaneous) Aquatic Science Virus Microbiology Cell Line 03 medical and health sciences Fish Diseases Immunity Goldfish medicine Carassius auratus Animals Carp Disease Resistance Cyprinid herpesvirus biology Inoculation Ecology DNA Viruses Water 04 agricultural and veterinary sciences biology.organism_classification DNA Virus Infections 030104 developmental biology Water temperature 040102 fisheries 0401 agriculture forestry and fisheries sense organs Disease Susceptibility medicine.symptom |
| Zdroj: | Journal of fish diseases. 40(2) |
| ISSN: | 1365-2761 |
| Popis: | Herpesviral haematopoietic necrosis (HVHN), caused by cyprinid herpesvirus-2 (CyHV-2), has affected the commercial production of the goldfish Carassius auratus and gibelio carp Carassius auratus gibelio. High water temperature treatments are reported to reduce the mortality rate of infected goldfish and elicit immunity in the survivors. To define the mechanism by which this intervention induces resistance, clonal ginbuna Carassius auratus langsdorfii, which is closely related to both species and has been used in fish immunology, may represent a promising model species. In this study, we investigated the susceptibility of clonal ginbuna strains to CyHV-2 and the effect of high water temperature treatment on infected ginbuna and goldfish. Experimental intraperitoneal infection with CyHV-2 at 25 °C caused 100% mortality in ginbuna strains, which was accompanied by histopathological changes typical of HVHN. Both infected ginbuna S3n strain and goldfish, exposed to high temperature for 6 days [shifting from 25 °C (permissive) to 34 °C (non-permissive)], showed reduced mortalities after the 1st inoculation, and subsequent 2nd virus challenge to 0%, indicating induction of immunity. It was concluded that ginbuna showed a similar susceptibility and disease development in CyHV-2 infection compared to goldfish, suggesting that ginbuna can be a useful fish model for the study of CyHV-2 infection and immunity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text