Human recombinant alkaline phosphatase inhibits ex vivo platelet activation in humans
| Autor: | Peter Pickkers, P. G. De Groot, A.J.A.M. van der Ven, Rahajeng N. Tunjungputri, Esther Peters, Q. de Mast |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Blood Platelets Male 0301 basic medicine Platelet Aggregation lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] 030204 cardiovascular system & hematology Pharmacology Lipopeptides 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Sepsis medicine Humans Platelet Platelet activation Aged Whole blood business.industry Hematology Acute Kidney Injury Middle Aged Alkaline Phosphatase Platelet Activation Adenosine Recombinant Proteins Adenosine Diphosphate Adenosine diphosphate Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11] lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4] 030104 developmental biology chemistry Immunology Alkaline phosphatase Platelet aggregation inhibitor Female Carrier Proteins Peptides business Platelet Aggregation Inhibitors Ex vivo medicine.drug |
| Zdroj: | Thrombosis and Haemostasis, 116, 1111-1121 Thrombosis and Haemostasis, 116, 6, pp. 1111-1121 Europe PubMed Central |
| ISSN: | 2567-689X 0340-6245 |
| DOI: | 10.1160/th16-03-0206 |
| Popis: | Contains fulltext : 172714.pdf (Publisher’s version ) (Closed access) Sepsis-associated acute kidney injury (AKI) is associated with high morbidity and mortality. Excessive platelet activation contributes to AKI through the formation of microthrombi and amplification of systemic inflammation. Two phase II trials demonstrated that bovine-intestinal alkaline phosphatase (AP) improved renal function in critically ill patients with sepsis-associated AKI. In this study, we characterised the platelet-inhibiting effects of a human recombinant AP. Whole blood and platelet-rich plasma (PRP) of healthy volunteers (n=6) was pre-treated ex vivo with recAP, whereafter platelet reactivity to ADP, collagen-related peptide (CRP-XL) and Pam3CSK4 was determined by flow cytometry. RecAP (40 U/ml) reduced the platelet reactivity to ADP (inhibition with a median of 47 %, interquartile range 43-49 %; p |
| Databáze: | OpenAIRE |
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