The effects of tricaine mesylate on arthropods: crayfish, crab and Drosophila
| Autor: | Alexa Larson, Ibraheem Murtaza, Ellora Amrit, Jeremy Nadolski, Courtney K. M. Waterbury, Destaneh Nichols, Carson Hill, Robin L. Cooper, Catherine E. Bohnett, Sabbyasachi Dhar, Keegan Deweese, Katrina Kozak, Morgan Inks, Hannah Tyger, Kelsey Campbell, Catherine E. Stanley, Rebecca Adams, Matthew Barrett, Barbara Gillis, Rafael Roberts |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Tricaine mesylate Callinectes Brachyura Neuromuscular Junction Action Potentials Sensory system Astacoidea Neurotransmission Synaptic Transmission Neuromuscular junction 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Developmental Neuroscience medicine Animals Aminobenzoates Procambarus clarkii Motor Neurons biology biology.organism_classification Crayfish Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Drosophila Drosophila melanogaster 030217 neurology & neurosurgery |
| Zdroj: | Invertebrate neuroscience : IN. 20(3) |
| ISSN: | 1439-1104 |
| Popis: | Tricaine mesylate, also known as MS-222, was investigated to characterize its effects on sensory neurons, synaptic transmission at the neuromuscular junction, and heart rate in invertebrates. Three species were examined: Drosophila melanogaster, blue crab (Callinectes sapidus), and red swamp crayfish (Procambarus clarkii). Intracellular measures of action potentials in motor neurons of the crayfish demonstrated that MS-222 dampened the amplitude, suggesting that voltage-gated Na + channels are blocked by MS-222. This is likely the mechanism behind the reduced activity measured in sensory neurons and depressed synaptic transmission in all three species as well as reduced cardiac function in the larval Drosophila. To address public access to data, a group effort was used for analysis of given data sets, blind to the experimental design, to gauge analytical accuracy. The determination of a threshold in analysis for measuring extracellular recorded sensory events is critical and is not easily performed with commercial software. |
| Databáze: | OpenAIRE |
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