Effects of overexpression of STB5 in Saccharomyces cerevisiae on fatty acid biosynthesis, physiology and transcriptome
| Autor: | Verena Siewers, John Hellgren, Jens Nielsen, Yun Chen, Dóra Vitay, Alexandra Linda Bergman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae Oxidative phosphorylation Biology Pentose phosphate pathway fatty acids Microbiology Applied Microbiology and Biotechnology Metabolic engineering Transcriptome chemistry.chemical_compound Biosynthesis Gene Expression Regulation Fungal NADPH transcription factor chemistry.chemical_classification STB5 Fatty Acids Fatty acid General Medicine biology.organism_classification chemistry Biochemistry metabolic engineering Flux (metabolism) Gene Deletion Research Article Transcription Factors |
| Zdroj: | Bergman, A, Vitay, D, Hellgren, J, Chen, Y, Nielsen, J & Siewers, V 2019, ' Effects of overexpression of STB5 in Saccharomyces cerevisiae on fatty acid biosynthesis, physiology and transcriptome ', FEMS Yeast Research, vol. 19, no. 3 . https://doi.org/10.1093/femsyr/foz027 FEMS Yeast Research |
| DOI: | 10.1093/femsyr/foz027 |
| Popis: | Microbial conversion of biomass to fatty acids (FA) and products derived thereof is an attractive alternative to the traditional oleochemical production route from animal and plant lipids. This study examined if NADPH-costly FA biosynthesis could be enhanced by overexpressing the transcription factor Stb5 in Saccharomyces cerevisiae. Stb5 activates expression of multiple genes encoding enzymes within the pentose phosphate pathway (PPP) and other NADPH-producing reactions. Overexpression of STB5 led to a decreased growth rate and an increased free fatty acid (FFA) production during growth on glucose. The improved FFA synthetic ability in the glucose phase was shown to be independent of flux through the oxidative PPP. RNAseq analysis revealed that STB5 overexpression had wide-ranging effects on the transcriptome in the batch phase, and appeared to cause a counterintuitive phenotype with reduced flux through the oxidative PPP. During glucose limitation, when an increased NADPH supply is likely less harmful, an overall induction of the proposed target genes of Stb5 (eg. GND1/2, TAL1, ALD6, YEF1) was observed. Taken together, the strategy of utilizing STB5 overexpression to increase NADPH supply for reductive biosynthesis is suggested to have potential in strains engineered to have strong ability to consume excess NADPH, alleviating a potential redox imbalance. STB5 —encoding a transcriptional activator of genes related to NADPH biosynthesis — was overexpressed in an attempt to enhance FA biosynthesis and the resulting effects were studied at different growth conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|