Ischemic Preconditioning Preventing Downregulation of miR-182 Protects Intestine Against Ischemia/Reperfusion Injury by Inhibiting Apoptosis
| Autor: | Lijuan Bian, Xiaoyun Duan, Yunsheng Li, Liting Kuang, Yanhua Luo, Zhen Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Ischemia Down-Regulation Apoptosis Pharmacology 03 medical and health sciences Mice 0302 clinical medicine Downregulation and upregulation Intestinal mucosa parasitic diseases microRNA medicine Animals cardiovascular diseases Ischemic Preconditioning business.industry General Medicine medicine.disease Intestines MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Reperfusion Injury Ischemic preconditioning Diamine oxidase business Reperfusion injury |
| Zdroj: | Archives of medical research. 50(5) |
| ISSN: | 1873-5487 |
| Popis: | Background Intestinal ischemia/reperfusion (I/R) injury is a severe condition associated with high morbidity and mortality. Ischemic preconditioning (IPC) had been found to be the most promising strategies against I/R injury. However, the potential molecular mechanisms underlying the protective effect of IPC have not been fully disclosed. MicroRNA182 (miR-182) is closely related to apoptosis and plays an important role in I/R injury. Our recent study demonstrated that miR-182 was down-regulated in the intestinal mucosa after I/R injury. However, whether miR-182 is involved in the protective effects of IPC in the setting of intestinal I/R injury is unknown. Aims To investigate the role of miR-182 in the protective effect of IPC in intestine after I/R injury and potential mechanisms. Methods AntagomiR-182 was pretreated before IPC in mice with intestinal I/R injury. MiR-182 mimic was administered before oxygen and glucose deprivation and reperfusion (OGD/R) in mice intestinal mucosa epithelial (MIME) cells. Results IPC partially prevented the downregulation of miR-182 in mice, which was blocked by pretreatment with antagomiR-182. Compared with the IPC group, pretreatment with antagomiR-182 further increased Chiu's scores and diamine oxidase activities. Meanwhile, apoptotic cells and cleaved caspase-3 expression were increased. Compared with the OGD/R group, pretreatment with miR-182 mimic prevented the downregulation of miR-182, improved cell survival, reduced apoptosis and cleaved caspase-3 expression in MIME cells. Conclusions The downregulation of miR-182 was partially prevented by IPC, which was involved in IPC induced intestinal protection, and the mechanisms may be associated with inhibition of apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect