Increased total scavenger capacity and decreased liver fat content in rats fed dehydroepiandrosterone and its sulphate on a high-fat diet
| Autor: | György Illyés, Zoltan Magyar, Dezso Szombath, Istvan Marczell, János Fehér, János Rigó, Gabriella Lengyel, András Hrabák, Béla Székács, Károly Rácz, Anna Blázovics, Gabor Bekesi, Zsuzsa Schaff, Elek Dinya, Peter Gergics, Zsolt Tulassay |
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| Rok vydání: | 2009 |
| Předmět: |
Male
endocrine system Aging medicine.medical_specialty Antioxidant Normal diet medicine.medical_treatment Dehydroepiandrosterone Administration Oral Antioxidants Superoxide dismutase Rats Sprague-Dawley chemistry.chemical_compound Random Allocation Oral administration Reference Values Internal medicine polycyclic compounds medicine Animals skin and connective tissue diseases Glutathione Transferase biology Dehydroepiandrosterone Sulfate Superoxide Dismutase Glutathione Catalase Dietary Fats Rats Fatty Liver Disease Models Animal Endocrinology Glutathione S-transferase chemistry Spectrophotometry biology.protein Geriatrics and Gerontology human activities hormones hormone substitutes and hormone antagonists |
| Zdroj: | Gerontology. 57(4) |
| ISSN: | 1423-0003 |
| Popis: | Background: Weak androgens have an antioxidant effect in vitro which is represented as a beneficial change in the antioxidant status. Objective: Our aim was to clarify whether dehydroepiandrosterone (DHEA) and dehydroepiandrosterone-sulphate (DHEAS) oral administration results in beneficial antioxidant changes in Sprague-Dawley adult male rats in vivo. Methods: Groups of experimental animals were fed a high-fat or a normal-fat diet and treated with DHEA or DHEAS in the drinking fluid. The control group was fed a high-fat diet together with untreated drinking fluid. Total scavenger capacity (TSC) was measured before and after 4 weeks of treatment in blood samples using a chemiluminometric assay. Fat content, superoxide dismutase (SOD), catalase and glutathione S-transferase (GST) activity in the liver were determined by Sudan staining and spectrophotometric assessments, respectively, from the fresh frozen tissue. Results: DHEA and the DHEAS treatment showed significantly increased TSC in the groups fed a high-fat diet. The control group and the DHEA- or DHEAS-treated groups on normal diets showed no significant changes in TSC. The total score of liver fat content in the high-fat diet groups showed a marked positivity with Sudan staining, and the groups treated with DHEA or DHEAS had a markedly decreased amount of fat in the liver slides compared to the untreated group on the high-fat diet. Liver SOD activity was decreased in all high-fat diet groups and elevated only in the groups on a normal diet with DHEA or DHEAS treatment. Liver catalase and GST activities were decreased in the groups where TSC was significantly increased. Conclusion: Our results support the hypothesis that DHEA and DHEAS supplementation can improve the antioxidant status in lipid-rich dietary habits. |
| Databáze: | OpenAIRE |
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