Sympatric diploid and tetraploid cytotypes of Centaurea stoebe s.l. do not differ in arbuscular mycorrhizal communities and mycorrhizal growth response
| Autor: | Jana Rydlová, Jan Suda, Jana Voříšková, Patrik Mráz, Heinz Müller-Schärer, Zuzana Kolaříková, Stanislav Španiel, Radka Sudová, Petr Kohout |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Population Centaurea Plant Science 010603 evolutionary biology 01 natural sciences Glomeraceae Mycorrhizae Botany Genetics Stoebe education Fertilizers Ecology Evolution Behavior and Systematics Herbivore education.field_of_study Centaurea maculosa biology fungi 15. Life on land biology.organism_classification Diploidy Tetraploidy Sympatric speciation Ploidy Centaurea stoebe 010606 plant biology & botany |
| Zdroj: | American journal of botany. 105(12) |
| ISSN: | 1537-2197 |
| Popis: | Premise of the study Genome duplication is associated with multiple changes at different levels, including interactions with pollinators and herbivores. Yet little is known whether polyploidy may also shape belowground interactions. Methods To elucidate potential ploidy-specific interactions with arbuscular mycorrhizal fungi (AMF), we compared mycorrhizal colonization and assembly of AMF communities in roots of diploid and tetraploid Centaurea stoebe s.l. (Asteraceae) co-occurring in a Central European population. In a follow-up greenhouse experiment, we tested inter-cytotype differences in mycorrhizal growth response by combining ploidy, substrate, and inoculation with native AMF in a full-factorial design. Key results All sampled plants were highly colonized by AMF, with the Glomeraceae predominating. AMF-community composition revealed by 454-pyrosequencing reflected the spatial distribution of the hosts, but not their ploidy level or soil characteristics. In the greenhouse experiment, the tetraploids produced more shoot biomass than the diploids did when grown in a more fertile substrate, while no inter-cytotype differences were found in a less fertile substrate. AMF inoculation significantly reduced plant growth and improved P uptake, but its effects did not differ between the cytotypes. Conclusions The results do not support our hypotheses that the cytotype structure in a mixed-ploidy population of C. stoebe is mirrored in AMF-community composition and that ploidy-specific fungal communities contribute to cytotype co-existence. Causes and implications of the observed negative growth response to AMF are discussed. |
| Databáze: | OpenAIRE |
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