Raman spectroscopy–based water measurements identify the origin of MRI T2 signal in human articular cartilage zones and predict histopathologic score
| Autor: | Corey P. Neu, Ozan Akkus, Robert L. Wilson, Mustafa Unal |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cartilage
Articular General Physics and Astronomy Articular cartilage Osteoarthritis Spectrum Analysis Raman Article General Biochemistry Genetics and Molecular Biology symbols.namesake medicine Humans General Materials Science medicine.diagnostic_test business.industry Human cartilage Cartilage General Engineering Water Magnetic resonance imaging General Chemistry medicine.disease Magnetic Resonance Imaging medicine.anatomical_structure symbols Cadaveric spasm business Nuclear medicine Raman spectroscopy |
| Zdroj: | J Biophotonics |
| ISSN: | 1864-0648 1864-063X |
| DOI: | 10.1002/jbio.202100212 |
| Popis: | We investigated for the first time zonal-dependent water distribution in articular cartilage by Raman spectroscopy (RS). We further investigated the association of histopathologic score with RS- and magnetic resonance imaging (MRI)-based water measurements. Cadaveric human cartilage plugs (N = 16) with different osteoarthritis (OA) severity were used. Water content distribution in cartilage zones was probed using RS- and MRI-based techniques. Histopathologic scoring was performed by two independent observers blindly. Moderate associations existed between RS- and MRI-based water measurements across all cartilage zones. RS-based analysis of different water compartments helped assign the origin of the T2 signal collected from the various cartilage zones. RS-based water parameters significantly correlated with OA-severity score, whereas MRI-based water measurements did not. RS can probe different water compartments in cartilage zones and predict up to 66% of the variation observed in the histopathologic score. RS-based water measurement could be developed further to assess cartilage quality in the clinic. |
| Databáze: | OpenAIRE |
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