Adenoviral targeting of malignant melanoma for fluorescence-guided surgery prevents recurrence in orthotopic nude-mouse models
| Autor: | Kiyoto Takehara, Toshiyoshi Fujiwara, Robert M. Hoffman, Shunsuke Kagawa, Michael Bouvet, Shuya Yano, Hiroyuki Kishimoto, Yasuo Urata |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pathology Residual cancer Melanoma Experimental Mice Nude medicine.disease_cause Complete resection Fluorescence Green fluorescent protein Resection Adenoviridae 03 medical and health sciences Mice 0302 clinical medicine Nude mouse Cell Line Tumor melanoma Medicine Animals Humans biology business.industry Melanoma Optical Imaging OBP-401 Dual effect adenovirus medicine.disease biology.organism_classification nude mice 3. Good health Surgery Disease Models Animal 030104 developmental biology Oncology 030220 oncology & carcinogenesis Neoplasm Recurrence Local business fluorescence-guided surgery (FGS) Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Malignant melanoma requires precise resection in order to avoid metastatic recurrence. We report here that the telomerase-dependent, green fluorescent protein (GFP)-containing adenovirus OBP-401 could label malignant melanoma with GFP in situ in orthotopic mouse models. OBP-401-based fluorescence-guided surgery (FGS) resulted in the complete resection of malignant melanoma in the orthotopic models, where conventional bright-light surgery (BLS) could not. High-dose administration of OBP-401 enabled FGS without residual cancer cells or recurrence, due to its dual effect of cancer-cell labeling with GFP and killing. |
| Databáze: | OpenAIRE |
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