Evidence for an intracellular precursor for human B-cell growth factor
| Autor: | Shashikant Mehta, Abby L. Maizel, Barbara Martin, David C. Wright, Surendra Sharma, Chintaman G. Sahasrabuddhe, John W. Morgan |
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| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
medicine.medical_treatment
Xenopus Biology Xenopus laevis Cytosol medicine Protein biosynthesis Animals Humans Trypsin RNA Messenger Protein Precursors Growth Substances Messenger RNA B-Lymphocytes Lymphokines Multidisciplinary Growth factor Lymphokine Biological activity biology.organism_classification Peptide Fragments Molecular Weight Kinetics Biochemistry Protein Biosynthesis RNA Interleukin-4 Poly A Intracellular Research Article |
| Popis: | Human B-cell growth factor has been described as a trypsin-sensitive protein of Mr 12,000-14,000. Evidence is provided herein that this relatively low molecular weight product may be released from a larger precursor molecule of Mr 60,000-80,000. The precursor protein is confined to the cytosol of freshly isolated T lymphocytes, and only the Mr 12,000-14,000 moiety is released upon lectin stimulation. The precursor protein was subjected to limited tryptic digestion, which demonstrated that the biologically active fraction of the moiety resided in a relatively low molecular weight fragment. The T lymphocyte routinely possessed an intracytoplasmic pool of the precursor protein, the amount of which cyclically varied depending upon its depletion by the secretion process of a lower molecular weight product. Analysis of the mRNA size coding for the majority of B-cell growth factor activity, determined by translation in Xenopus laevis oocytes, suggested that the B-cell growth factor-specific mRNA resided in the greater than or equal to 15S range. This value is consistent with the size of the larger precursor. Therefore, it is proposed that a precursor-product relationship exists for the processing of human B-cell growth factor, analogous to that which has been described for several other cytokines. |
| Databáze: | OpenAIRE |
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