Characterization of visna virus mRNA
Autor: | P Filippi, J Tamalet, R Vigne, Michel Brahic |
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Rok vydání: | 1979 |
Předmět: |
Cytoplasm
Visna-maedi virus Visna virus viruses Immunology Microbiology Cell Line Retrovirus Virology Polysome Animals RNA Messenger Polyacrylamide gel electrophoresis Messenger RNA Sheep biology RNA biology.organism_classification Molecular biology Molecular Weight Cell culture Polyribosomes Insect Science Choroid Plexus RNA Viral Poly A Research Article |
Zdroj: | Journal of Virology. 31:25-30 |
ISSN: | 1098-5514 0022-538X |
DOI: | 10.1128/jvi.31.1.25-30.1979 |
Popis: | Visna virus is a retrovirus responsible for a classical slow infection of the central nervous system of sheep. In the present work we focused our attention on the viral mRNA's. We found that, during the acute infection in vitro, (i) viral mRNA's amount to only 0.1% of the total cytoplasmic RNA, (ii) 20% of the total cytoplasmic viral RNA is found in polyribosomes, and (iii) three viral mRNA's can be identified by sucrose gradient sedimentation or polyacrylamide gel electrophoresis. Their sedimentation coefficients are 36S, 27S, and 21S. |
Databáze: | OpenAIRE |
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