Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept
| Autor: | Magali Chabé, Vincent Chieux, Baptiste Delaire, Karine Guyot, Sadia Benamrouz Vanneste, Jérôme Follet, Martha Baydoun, Anthony Mouray, Vincent Senez, Atallah Baydoun, Gerard Forzy, Gabriela Certad, Nausicaa Gantois, Colette Creusy, Pierre Gosset, Eric Viscogliosi |
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| Přispěvatelé: | Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Institut Supérieur d'Agriculture de Lille (ISA), Université catholique de Lille (UCL), Écologie et Biodiversité, Smart and Sustainable Cities (SCC), Université Catholique de Lille - Faculté de gestion, économie et sciences (FGES), Institut Catholique de Lille (ICL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Institut Catholique de Lille (ICL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université Catholique de Lille - Faculté de gestion, économie et sciences (FGES), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Groupement des Hôpitaux de l'Institut Catholique de Lille (GHICL), Plateforme d'Expérimentations et de Hautes Technologies Animales [Lille], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), ROOS, Clélia |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Colon 030106 microbiology Cell Cell Culture Techniques Cryptosporidiosis lcsh:Medicine Mice SCID In Vitro Techniques medicine.disease_cause Article Host-Parasite Interactions Pathogenesis Mice 03 medical and health sciences [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases parasitic diseases medicine Animals Humans lcsh:Science Cell Proliferation Cryptosporidium parvum Multidisciplinary biology Cell growth lcsh:R Cryptosporidium biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology In vitro Disease Models Animal 030104 developmental biology medicine.anatomical_structure Colonic Neoplasms Cancer research [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases lcsh:Q [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Carcinogenesis Ex vivo Signal Transduction |
| Zdroj: | Scientific Reports Scientific Reports, 2017, 7 (1), pp.17288. ⟨10.1038/s41598-017-17304-2⟩ Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-17304-2⟩ |
| Popis: | Cryptosporidium parvum is a major cause of diarrheal illness and was recently potentially associated with digestive carcinogenesis. Despite its impact on human health, Cryptosporidium pathogenesis remains poorly known, mainly due to the lack of a long-term culture method for this parasite. Thus, the aim of the present study was to develop a three-dimensional (3D) culture model from adult murine colon allowing biological investigations of the host-parasite interactions in an in vivo-like environment and, in particular, the development of parasite-induced neoplasia. Colonic explants were cultured and preserved ex vivo for 35 days and co-culturing was performed with C. parvum. Strikingly, the resulting system allowed the reproduction of neoplastic lesions in vitro at 27 days post-infection (PI), providing new evidence of the role of the parasite in the induction of carcinogenesis. This promising model could facilitate the study of host-pathogen interactions and the investigation of the process involved in Cryptosporidium-induced cell transformation. |
| Databáze: | OpenAIRE |
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