Differential effects of peroxynitrite on the function of arginine vasopressin V1a receptors and alpha1-adrenoceptors in vivo
| Autor: | Neil W. Kooy, Stephen J. Lewis, James N. Bates, Tom P. Robertson, Kevin Sandock, Azizul Hoque |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Receptors Vasopressin medicine.medical_specialty Vasopressin Time Factors Vascular smooth muscle Physiology Blood Pressure Muscle Smooth Vascular Rats Sprague-Dawley Phenylephrine chemistry.chemical_compound Renal Artery Mesenteric Artery Superior Peroxynitrous Acid Receptors Adrenergic alpha-1 Arginine vasopressin receptor 2 Internal medicine medicine Animals Vasoconstrictor Agents Aorta Abdominal Vasopressin receptor Pharmacology Arginine vasopressin receptor 1B Nitrates Nitroblue Tetrazolium Glutathione Rats Arginine Vasopressin Peroxynitrous acid Endocrinology chemistry Vasoconstriction Hydroxymercuribenzoates Molecular Medicine Vascular Resistance Oxidation-Reduction hormones hormone substitutes and hormone antagonists Peroxynitrite medicine.drug |
| Zdroj: | Vascular Pharmacology. 46:24-34 |
| ISSN: | 1537-1891 |
| DOI: | 10.1016/j.vph.2006.06.004 |
| Popis: | Objective The aim of this study was to provide evidence that peroxynitrite may differentially affect the function of arginine vasopressin (AVP) V1a receptors and α1-adrenoceptors in vascular smooth muscle of the rat Methods The vasoconstrictor responses elicited by AVP, or the α1-adrenoceptor agonist, phenylephrine, were determined in anesthetized rats before and after injections of (i) peroxynitrite, the thiol chelator, para-hydroxymercurobenzoic acid (PHMBA), or the electron acceptor, nitroblue tetrazolium (NBT). The ability of the reducing agent, glutathione, to reverse the loss of response to phenylephrine and AVP in peroxynitrite-treated rats was also examined. Results The AVP-induced responses were suppressed 10–20 min but not 60–70 min after the administration of peroxynitrite. Glutathione reversed the above loss of response to AVP at 10–20 min. The responses elicited by phenylephrine were suppressed 10–20 min and 60–70 min after administration of peroxynitrite. Glutathione did not reverse the above losses of response to phenylephrine. In addition, the vasoconstrictor actions of AVP and phenylephrine were markedly suppressed after administration of PHMBA or nitroblue tetrazolium. Conclusions The above findings provide evidence that exogenously administered peroxynitrite may differentially affect the function of AVP V1a receptors and α1-adrenoceptors in vascular smooth muscle of the rat. The possibility that peroxynitrite impairs AVP V1a receptor function by transient oxidation events whereas peroxynitrite impairs α1-adrenoceptor function by transient oxidation and permanent nitration events will be discussed. |
| Databáze: | OpenAIRE |
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