Nitric Oxide and Nitric Oxide Synthase mRNA Induction in Mouse Islet Cells by Interferon-γ Plus Tumor Necrosis Factor-α
| Autor: | Kyohei Nonaka, Kentaro Yamada, Shuichi Otabe, Chizuko Inada, Naoko Takane |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.medical_treatment Molecular Sequence Data Biophysics Cycloheximide Biology Arginine Nitric Oxide Biochemistry Nitric oxide Interferon-gamma Islets of Langerhans Mice chemistry.chemical_compound Internal medicine medicine Animals Interferon gamma RNA Messenger Molecular Biology Cell damage Cells Cultured geography omega-N-Methylarginine geography.geographical_feature_category Base Sequence Tumor Necrosis Factor-alpha Cell Biology Islet medicine.disease Molecular biology Nitric oxide synthase Endocrinology Cytokine chemistry Enzyme Induction biology.protein Cytokines Tumor necrosis factor alpha Amino Acid Oxidoreductases Nitric Oxide Synthase medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 197:22-27 |
| ISSN: | 0006-291X |
| Popis: | It has been shown that nitric oxide (NO) is involved in islet cell damage induced by interleukin-1 (IL-1). Here we show that interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) synergistically induced NO production and inducible NO synthase (iNOS) mRNA expression in mouse islet cells. Cycloheximide (CXH) did not prevent the iNOS mRNA expressions. The combination of IFN-gamma and TNF-alpha, which is highly cytotoxic to mouse islet cells, failed to destruct islet cells in the absence of L-arginine or in the presence of NG-monomethyl-L-arginine (NMMA). These observations suggest that NO is a primary effector in islet cell damage caused by IFN-gamma plus TNF-alpha. |
| Databáze: | OpenAIRE |
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