Boronic Acid-Functionalized Core–Shell–Shell Magnetic Composite Microspheres for the Selective Enrichment of Glycoprotein
| Autor: | Jin Zheng, Miaorong Pan, Yangfei Sun, Wuli Yang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Morphology (linguistics)
Materials science Polymers Composite number Emulsion polymerization Methacrylate Horseradish peroxidase Magnetics chemistry.chemical_compound Animals Organic chemistry General Materials Science Horseradish Peroxidase biology Serum Albumin Bovine Hydrogen-Ion Concentration Silicon Dioxide Boronic Acids Ferrosoferric Oxide Microspheres chemistry Chemical engineering Covalent bond biology.protein Magnetic nanoparticles Cattle Electrophoresis Polyacrylamide Gel Boronic acid |
| Zdroj: | ACS Applied Materials & Interfaces. 5:8351-8358 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | In this work, core-shell-shell-structured boronic acid-functionalized magnetic composite microspheres Fe3O4@SiO2@poly (methyl methacrylate-co-4-vinylphenylbornoic acid) (Fe3O4@SiO2@P(MMA-co-VPBA)) with a uniform size and fine morphology were synthesized. Here, Fe3O4 magnetic particles were prepared by a solvothermal reaction, whereas the Fe3O4@SiO2 microspheres with a core-shell structure were obtained by a sol-gel process. 3-(Trimethoxysilyl) propyl methacrylate (MPS)-modified Fe3O4@SiO2 was used as the seed in the emulsion polymerization of MMA and VPBA to form the core-shell-shell-structured magnetic composite microspheres. As the boronic acid groups on the surface of Fe3O4@SiO2@P(MMA-co-VPBA) could form tight yet reversible covalent bonds with the cis-1,2-diols groups of glycoproteins, the magnetic composite microspheres were applied to enrich a standard glycoprotein, horseradish peroxidase (HRP), and the results demonstrated that the composite microspheres have a higher affinity for the glycoproteins in the presence of the nonglycoprotein bovine serum albumin (BSA) over HRP. Additionally, different monomer mole ratios of MMA/VPBA were studied, and the results implied that using MMA as the major monomer could reduce the amount of VPBA with a similar glycoprotein enrichment efficiency but a lower cost. |
| Databáze: | OpenAIRE |
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