The use of tannic acid for the ultrastructural visualization of hyaluronic acid
| Autor: | Michael Solursh, Carl T. Singley |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Histology
Polymers Hyaluronoglucosaminidase macromolecular substances Chick Embryo Fibril Glycosaminoglycan Extracellular matrix chemistry.chemical_compound Fixatives Hyaluronidase Formaldehyde Tannic acid Hyaluronic acid medicine Animals Wings Animal Hyaluronic Acid Molecular Biology Fixative Glycosaminoglycans Histocytochemistry Chondroitin Sulfates Cell Biology General Medicine Osmium Hydrolyzable Tannins Medical Laboratory Technology chemistry Biochemistry Glutaral Ultrastructure Collagen Anatomy General Agricultural and Biological Sciences Extracellular Space Tannins medicine.drug |
| Zdroj: | Histochemistry. 65(2) |
| ISSN: | 0301-5564 |
| Popis: | This study describes a method, which makes use of tannic acid (2%) as a component of a paraformaldehyde-glutaraldehyde based fixative, to reveal the presence and ultrastructure of glycosaminoglycans in the extracellular matrix. The ultrastructure of the extracellular matrix in the stage 24 chick embryo wing is examined after fixation by several procedures. After fixation in the absence of tannic acid, the intercellular spaces contain little extracellular matrix, except for occasional fibrils (collagen?). On the other hand, when tannic acid is included in the primary fixative, the intercellular spaces contain considerable amounts extracellular matrix which includes 3±0.5 nm filaments, ±30 nm granules, as well as putative collagen fibrils. The 3±0.5 nm diameter fibrils are not observed when the limbs had been injected in ovo with Streptomyces hyaluronidase (specific for hyaluronic acid) prior to fixation. Furthermore, the 3±0.5 nm fibrils resemble authentic hyaluronic acid that had been fixed by the same procedure in the presence of tannic acid. Limbs treated with tannic acid after osmication contained only small amounts of extracellular material, which was confined largely to cell surfaces. These results demonstrate that the use of tannic acid in the primary fixative can serve as a useful method for the ultrastructural visualization of several extracellular matrix materials, including hyaluronic acid. |
| Databáze: | OpenAIRE |
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