Preservation of nasopharyngeal smears for fluorescent antibody detection of Bordetella pertussis
| Autor: | B M Thomason, R M McKinney, P P Harris |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Microbiology (medical)
Serotype Bordetella pertussis biology Preservation Biological Fluorescent Antibody Technique biology.organism_classification Fluorescence Microbiology Staining Aprotinin Antigen Formaldehyde Nasopharynx medicine Humans Fixation (histology) Antibody detection medicine.drug Research Article |
| Zdroj: | Journal of clinical microbiology. 12(6) |
| ISSN: | 0095-1137 |
| Popis: | Smears from throat and nasal washings seeded with Bordetella pertussis were either treated with aprotinin (a protease inhibitor) or fixed with 1 or 10% Formalin. These smears were stored at 23, 4, and -70 degrees C. Smears were removed and stained with a fluorescent antibody conjugate for B. pertussis at intervals during 22 days of storage. Results indicate that treatment of smears with 4 or 8 U of aprotinin per ml preserved fluorescent antibody staining qualities of B. pertussis for 22 days; fixation with either concentration of Formalin was unsatisfactory. |
| Databáze: | OpenAIRE |
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