Effects of Extracted Cordyceps sinensis on Steroidogenesis in MA-10 Mouse Leydig Tumor Cells
| Autor: | Bu Miin Huang, Sew-Fen Leu, Chieu-Fu Chen, Yu Min Chuang |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_specialty Cell Pharmaceutical Science Cycloheximide Mice chemistry.chemical_compound Ascomycota Western blot Internal medicine Tumor Cells Cultured medicine Animals Progesterone Protein Synthesis Inhibitors Pharmacology Cordyceps Ethanol Leydig cell biology medicine.diagnostic_test Leydig Cells Water Biological activity General Medicine Phosphoproteins biology.organism_classification Molecular biology Stimulation Chemical In vitro Neoplasm Proteins medicine.anatomical_structure Endocrinology Bucladesine chemistry Leydig Cell Tumor Hypocreales Drugs Chinese Herbal |
| Zdroj: | Biological and Pharmaceutical Bulletin. 23:1532-1535 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.23.1532 |
| Popis: | Extracts from the mycelium of Cordyceps sinensis (CS) were tested to determine the in vitro effect on Leydig cell function. MA-10 mouse Leydig tumor cells were used to conduct the experiments. Results showed that progesterone production gradually increased as the dosage of combined water and ethanol extracted CS increased, and there was a statistically significant difference in progesterone production stimulated by 20 mg/ml of CS extracts compared to the control. The combined water and ethanol extracted CS significantly stimulated MA-10 cell steroid production at 12 and 24 h of incubation. In addition, a protein synthesis inhibitor, cycloheximide, did not block the stimulatory effects of CS extracts on MA-10 cell steroid production or total protein expression. Moreover, the expression of steroidogenic acute regulatory (StAR) protein, which is a critical protein for steroidogenesis, stimulated by CS extracts, could not be detected by Western blot analysis. These data indicate that CS extracts might not induce StAR protein and/or other protein expressions to stimulate steroidogenesis in MA-10 mouse Leydig tumor cells. |
| Databáze: | OpenAIRE |
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