Characterization of antioxidant system parameters in four freshwater fish species
| Autor: | Gülüzar Atli, Mustafa Canli, Esin G. Canli, Ali Eroglu |
|---|---|
| Přispěvatelé: | Çukurova Üniversitesi |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Carps food.ingredient Trout Characterization Health Toxicology and Mutagenesis Glutathione reductase Fresh Water 010501 environmental sciences 01 natural sciences Antioxidants Toxicology 03 medical and health sciences food Animals Food science Carp Catfishes Glutathione Transferase 0105 earth and related environmental sciences chemistry.chemical_classification Glutathione Peroxidase Freshwater fish biology Superoxide Dismutase Glutathione peroxidase Fishes Public Health Environmental and Occupational Health Tilapia General Medicine biology.organism_classification Glutathione Pollution Oxidative Stress Oreochromis Glutathione Reductase 030104 developmental biology Liver chemistry Antioxidant enzymes Environmental Monitoring Catfish |
| Zdroj: | Ecotoxicology and Environmental Safety. 126:30-37 |
| ISSN: | 0147-6513 |
| Popis: | PubMedID: 26707186 The potential use of antioxidant system parameters has gained considerable interest due to their pivotal role of detoxification mechanisms in environmental studies and culture fish point of view. Fish with different ecological needs may have different antioxidant capacity and response to environmental contaminants. Thus, the optimal working conditions and specific enzyme activities (Vmax and Km) of antioxidant system parameters (Superoxide dismutase, SOD; Catalase, CAT; Glutathione peroxidase, GPX; Glutathione reductase, GR and Glutathione S-transferase, GST) and glutathione (GSH) were determined in four commonly cultured freshwater fish species (tilapia; Oreochromis niloticus, carp; Cyprinus carpio, trout; Onchorhynchus mykiss and catfish; Clarias garipienus). Data showed that optimal concentrations of different buffers, pH and specific chemicals for each enzyme and GSH were similar in most cases for all fish species, except a few differences. The highest Vmax and Km values were found in carp for GPX and GST, though these values were the highest in tilapia, catfish and trout for CAT, SOD and GR, respectively. As a conclusion, optimization assays of these parameters in different bioindicator organisms based on their physiological and ecological differences may be useful for the aquatic ecosystem biomonitoring studies and also present fundamental data for utilization in aquaculture. © 2015 Elsevier Inc. IMYO2013BAP1 Çukurova Üniversitesi This study was supported by the Research Fund (IMYO2013BAP1) of Çukurova University . We thank Z. Dogan, E. Baysoy and K. Kocalar for their help during analysis. |
| Databáze: | OpenAIRE |
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