CD4 T cell help is required for primary CD8 T cell responses to vesicular antigen delivered to dendritic cellsin vivo

Autor: Laurent Giraudo, Karine Serre, Patrick Machy, Carole Siret, Lee Leserman
Přispěvatelé: Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)
Rok vydání: 2006
Předmět:
CD4-Positive T-Lymphocytes
Priming (immunology)
MESH: Flow Cytometry
CD8-Positive T-Lymphocytes
Lymphocyte Activation
MESH: Mice
Knockout
Mice
Immunology and Allergy
Cytotoxic T cell
MESH: Animals
IL-2 receptor
Mice
Knockout
MESH: Mice
Inbred CBA
MESH: Dendritic Cells
MESH: CD4-Positive T-Lymphocytes
CD28
Flow Cytometry
MESH: CD8-Positive T-Lymphocytes
Adoptive Transfer
MESH: Cell Growth Processes
MESH: Immunization
[SDV.IMM]Life Sciences [q-bio]/Immunology
MESH: Mice
Transgenic
Ovalbumin
Immunology
Antigen presentation
Mice
Transgenic
MESH: Cytotoxicity Tests
Immunologic
Cell Growth Processes
Biology
Antigen
MESH: Mice
Inbred C57BL
Animals
MESH: Lymphocyte Activation
Antigen-presenting cell
MESH: Mice
MESH: Ovalbumin
CD40
Dendritic Cells
Cytotoxicity Tests
Immunologic
Molecular biology
Mice
Inbred C57BL
MESH: Adoptive Transfer
MESH: Muramidase
Liposomes
Mice
Inbred CBA
biology.protein
MESH: Liposomes
Immunization
Muramidase
Zdroj: European Journal of Immunology
European Journal of Immunology, 2006, 36 (6), pp.1386-97. ⟨10.1002/eji.200526193⟩
European Journal of Immunology, Wiley-VCH Verlag, 2006, 36 (6), pp.1386-97. ⟨10.1002/eji.200526193⟩
ISSN: 1521-4141
0014-2980
Popis: Insight into the mechanisms by which dendritic cells (DC) present exogenous antigen to T cells is of major importance in the design of vaccines. We examined the effectiveness of free antigen as well as antigen with lipopolysaccharide, emulsified in complete Freund's adjuvant, and antigen encapsulated in liposomes in activating adoptively transferred antigen-specific CD4 and CD8 T cells. When contained in liposomes, 100- to 1000-fold lower antigen amounts were as efficient in inducing proliferation and effector functions of CD4 and CD8 T cells in draining lymph nodes as other antigen forms. CD11c(+)/CD11b(+)/CD205(mod)/CD8alpha(-) DC that captured liposomes were activated and presented this form of antigen in an MHC class I- and class II-restricted manner. CD4 T cells differentiated into Th1 and Th2 effector cells. Primary expansion and cytotoxic activity of CD8 T cells were CD4 T cell-dependent and required the transporter associated with antigen processing (TAP). Finally, adoptively transferred CD4 and CD8 T cells were not deleted after primary immunization and rapidly responded to a secondary immunization with antigen-containing liposomes. In conclusion, encapsulation of antigen in liposomes is an efficient way of delivering antigen to DC for priming of both CD4 and CD8 T cell responses. Importantly, primary CD8 T cell responses were CD4 T cell-dependent.
Databáze: OpenAIRE
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