Interaction of Fluorescently Substituted Metallacarboranes with Cyclodextrins and Phospholipid Bilayers: Fluorescence and Light Scattering Study
| Autor: | Pavel Matejicek, Piotr Jurkiewicz, Bohumír Grüner, Petr Cigler, Mariusz Uchman, Karel Procházka, Martin Hof |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular Light Lipid Bilayers Molecular Conformation Phospholipid Analytical chemistry Light scattering chemistry.chemical_compound Polymer chemistry Organometallic Compounds Electrochemistry Scattering Radiation General Materials Science Phospholipids Spectroscopy Fluorescent Dyes chemistry.chemical_classification Cyclodextrins Aqueous solution integumentary system Cyclodextrin Bilayer Cell Membrane Water Surfaces and Interfaces Condensed Matter Physics Fluorescence Spectrometry Fluorescence Membrane chemistry Self-assembly |
| Zdroj: | Langmuir. 26:6268-6275 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la904047k |
| Popis: | We prepared two fluorescein-[3-cobalt(III) bis(1,2-dicarbollide)](-) conjugates. They are sparingly soluble in water and form large aggregates in aqueous solutions. An extensive study on their spectral and aggregation behavior was carried out. To prepare their well-defined dispersion in aqueous systems, we studied the interaction of both probes with two biocompatible amphiphilic systems, cyclodextrins, which are frequently used in drug-delivery systems, and phospholipid membranes, which are the major constituents of cell barriers in living organisms. The presence of fluorescein in both conjugates allows us to study their behavior in detail by steady-state and time-resolved fluorometry, fluorescence correlation spectroscopy, and fluorescence lifetime imaging. The self-assembly of these metallacarboranes in aqueous solutions was studied by dynamic light scattering. The study shows that the compounds interact with cyclodextrins that increases their solubility in water, and they solubilize easily in phospholipid bilayers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|