Solid-state fermentation increases secretome complexity in Aspergillus brasiliensis
| Autor: | Alicia Chagolla-López, Daniel Salgado-Bautista, Ernesto Favela-Torres, Ulises Carrasco-Navarro, Francisco Figueroa-Martínez, Tania Volke-Sepúlveda |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Catabolite repression 01 natural sciences Fungal Proteins 03 medical and health sciences Tandem Mass Spectrometry Genetics Extracellular Amino Acids Ecology Evolution Behavior and Systematics 030304 developmental biology Analysis of Variance 0303 health sciences Aspergillus biology Nucleotides Aspergillus niger Carbon Dioxide Lipid Metabolism biology.organism_classification Oxidative Stress Glucose Infectious Diseases Secretory protein Biochemistry Solid-state fermentation Fermentation Carbohydrate Metabolism Electrophoresis Polyacrylamide Gel Energy Metabolism Oxidation-Reduction Transaldolase 010606 plant biology & botany |
| Zdroj: | Fungal Biology. 124:723-734 |
| ISSN: | 1878-6146 |
| DOI: | 10.1016/j.funbio.2020.04.006 |
| Popis: | Aspergillus is used for the industrial production of enzymes and organic acids, mainly by submerged fermentation (SmF). However, solid-state fermentation (SSF) offers several advantages over SmF. Although differences related to lower catabolite repression and substrate inhibition, as well as higher extracellular enzyme production in SSF compared to SmF have been shown, the mechanisms undelaying such differences are still unknown. To explain some differences among SSF and SmF, the secretome of Aspergillus brasiliensis obtained from cultures in a homogeneous physiological state with high glucose concentrations was analyzed. Of the regulated proteins produced by SmF, 74% were downregulated by increasing the glucose concentration, whereas all those produced by SSF were upregulated. The most abundant and upregulated protein found in SSF was the transaldolase, which could perform a moonlighting function in fungal adhesion to the solid support. This study evidenced that SSF: (i) improves the kinetic parameters in relation to SmF, (ii) prevents the catabolite repression, (iii) increases the branching level of hyphae and oxidative metabolism, as well as the concentration and diversity of secreted proteins, and (iv) favors the secretion of typically intracellular proteins that could be involved in fungal adhesion. All these differences can be related to the fact that molds are more specialized to growth in solid materials because they mimic their natural habitat. |
| Databáze: | OpenAIRE |
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