Inhibition of epidermal growth factor binding to cultured mouse epidermal cells by tumor promoters
| Autor: | Mario Froscio, Andrew W. Murray |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Cancer Research
TGF alpha Epidermal Growth Factor integumentary system Chemistry Promoter General Medicine Molecular biology ErbB Receptors Mice Epidermal Cells Covalent bond Epidermal growth factor Depression Chemical Phorbol Esters Epidermal growth factor binding Carcinogens Animals Tetradecanoylphorbol Acetate Growth factor receptor inhibitor Epidermis Receptor A431 cells hormones hormone substitutes and hormone antagonists Protein Binding |
| Zdroj: | Carcinogenesis. 1:681-684 |
| ISSN: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/1.8.681 |
| Popis: | The tumor promotor 12-O-tetradecanoylphorbol-13-acetate (TPA) caused partial inhibition of the binding of [125I]epidermal growth factor ([125I]EGF) to intact mouse epidermal cells (HEL/37). The proportion of [125I]EGF binding which was insensitive to TPA inhibition decreased with increasing EGF concentration. The partial inhibition of [125I]EGF binding was not due to destruction of TPA or to covalent linkage of EGF to receptor sites. The binding of [125I]EGF to HEL/37 cells showed a curvilinear Scatchard plot; this was converted into a linear plot in the presence of TPA. We conclude that TPA acts to inhibit interactions between EGF receptors or between EGF receptors and some other membrane component(s). |
| Databáze: | OpenAIRE |
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