Molecular epidemiology of hepatitis B virus in Amsterdam 1992-1997
| Autor: | J. E. van Steenbergen, R. A. Coutinho, Hubert G. M. Niesters, J. A. R. Van Den Hoek, E L M Op de Coul, A.D.M.E. Osterhaus, G. J. J. Van Doornum, A. Leentvaar-Kuijpers |
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| Přispěvatelé: | Infectious diseases, Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Male
Hepatitis B virus Molecular Sequence Data Population Biology medicine.disease_cause Risk-Taking Virology Genotype medicine Humans Genetic variability education Phylogeny Netherlands Retrospective Studies Genetics Hepatitis Molecular Epidemiology education.field_of_study Molecular epidemiology Phylogenetic tree Sequence Analysis DNA Middle Aged Hepatitis B medicine.disease Infectious Diseases Acute Disease DNA Viral Female |
| Zdroj: | Journal of medical virology, 66(2), 159-165. Wiley-Liss Inc. |
| ISSN: | 0146-6615 |
| DOI: | 10.1002/jmv.2125 |
| Popis: | To gain insight into the spread of hepatitis B among various risk groups in Amsterdam a 6-year (1992‐1997) retrospective DNA sequencing study was carried out on isolates from stored sera from reported primary cases of acute hepatitis B infection. Cases were classified according to risk behavior, as determined in interviews. Of the available serum, a selected region of hepatitis B-virus-DNA was amplified and sequenced. The nucleotide alignments were subjected to phylogenetic tree analysis. When nucleotide alignments were subjected to phylogenetic analysis, the strains of 54 isolates, 26% of the 204 reported primary cases, clustered in five genotypes: A, C, D, E, and F. In genotype A, a cluster related to men having sex with men was identified. In genotype D, two subclusters could be identified: one was related to injecting drug use and another was related to the Moroccan population in Amsterdam. The remaining strains showed a high genetic variability within three different genotypes: F, E, and C. Of the 14 identical isolates in the ‘‘homosexual men cluster,’’ one was isolated from a female heterosexual. Of the 14 identical strains in the ‘‘drug users strain,’’ six were from non-drug using heterosexual active individuals. In the cluster of twelve isolates related to hepatitis B-endemic areas, probable modes of transmission were varied. Sequence analysis provides important insight into the spread of hepatitis B among various highrisk groups. The analysis indicates that the prevention strategy in The Netherlands fails to stop transmission of hepatitis B from persistently infected individuals originating from hepatitis Bendemic countries. J. Med. Virol. 66:159‐ |
| Databáze: | OpenAIRE |
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