Profile of MicroRNA Expression in Brain Tumors of Different Malignancy
| Autor: | G L Kobyakov, A. N. Konovalov, D. A. Chistyakov, L V Shishkina, D. Yu. Usachyov, F. A. Koshkin, David I. Pitskhelauri, Alexey Nikitin, Vladimir P. Chekhonin, Alexander Potapov |
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| Rok vydání: | 2014 |
| Předmět: |
Diamines
Biology Real-Time Polymerase Chain Reaction Malignancy Statistics Nonparametric General Biochemistry Genetics and Molecular Biology Transcriptome chemistry.chemical_compound Glioma Biopsy microRNA Biomarkers Tumor medicine Humans Benzothiazoles Organic Chemicals DNA Primers Regulation of gene expression medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction General Medicine medicine.disease Gene Expression Regulation Neoplastic MicroRNAs Real-time polymerase chain reaction chemistry Quinolines SYBR Green I Cancer research |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 157:794-797 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-014-2669-8 |
| Popis: | The expression profiles of 10 mature microRNA (7, 10a, 17, 20a, 21, 23a, 26a, 137, 222) in biopsy specimens of gliomas of different malignancy were studied by the real time PCR with SYBR Green I fluorescent stain. The expression of microRNA-21 increased significantly, while that of microRNA-137 decreased, depending on the tumor malignancy. The expression of microRNA-9, -17, -20a, -23a, -26a was significantly higher, while that of microRNA-7 lower in the tumor vs. control tissue samples. New data on the molecular pathological mechanisms of gliomas related to their malignancy were obtained. Quantitative analysis of microRNA was suggested as a potential diagnostic marker of brain tumors. |
| Databáze: | OpenAIRE |
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