Screening of six medicinal plant species for antileishmanial activity
| Autor: | Ranulfo Piau Junior, Márcia Regina Pereira Cabral, Izabel Galhardo Demarchi, Thaís Gomes Verzignassi Silveira, Maria Helena Sarragiotto, Fabiana Borges Padilha Ferreira, Carla Maria Mariano Fernandez, Zilda Cristiani Gazim, Maria Valdrinez Campana Lonardoni |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pharmacology
0303 health sciences Traditional medicine hymenaea stigonocarpa leishmania braziliensis business.industry 030231 tropical medicine Pharmaceutical Science antileishmanial food and beverages Pharmacy General Medicine Biology Piper corcovadensis Hymenaea stigonocarpa Leishmania amazonensis Leishmania braziliensis cytotoxicity hemolysis piper corcovadensis 03 medical and health sciences 0302 clinical medicine leishmania amazonensis Plant species HD9665-9675 business Pharmaceutical industry 030304 developmental biology |
| Zdroj: | Acta Pharmaceutica Volume 71 Issue 3 Acta Pharmaceutica, Vol 71, Iss 3, Pp 399-414 (2021) |
| ISSN: | 1846-9558 1330-0075 |
| Popis: | This study is aimed to investigate the in vitro anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonocarpa and Piper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of Leishmania amazonensis and Leishmania braziliensis and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for L. amazonensis and L. braziliensis were shown by the ethanolic extract of the fruits of Hymenaea stigonocarpa and dichloromethane extract of the roots of Piper corcovadensis, with IC 50 of 160 and 150 μg mL−1, resp. Ethanolic extracts of A. muricata (leaf), B. uniflora (flower and leaf), M. pungens (fruit and leaf), N. megapotamica (leaf), and aqueous extract of H. stigonocarpa (fruit) showed IC 50 > 170 μg mL−1 for L. amazonensis and > 200 μg mL−1 for L. braziliensis. The extracts exhibited low cytotoxicity towards J774.A1 macrophages with CC 50 > 1000 μg mL−1 and hemolytic activity from 0 to 46.1 %. |
| Databáze: | OpenAIRE |
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